Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 209 |
| PDBID: | 2A0L |
| Chains: | FE_B |
| Organism: | Aeropyrum pernix, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.90 |
| Reference: | 10.1073/pnas.0507651102 |
| Antibody | |
| Antibody: | 33H1 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Voltage-gated potassium channel |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >2A0L_F|Chain D, F|33H1 Fv fragment|Mus musculus (10090) DVQLQESGPGLVKPSQSLSLTCTVTGYSITNNYAWNWIRQFPGNKLEWMGYINYSGTTSYNPSLKSRISITRDTSKNQFFLQLNSVTTEDTATYFCVRGYDYFAMDYWGQGTSVTV |
Light Chain: E
Mutation: NULL
| >2A0L_E|Chain C, E|33H1 Fv fragment|Mus musculus (10090) QIVLTQSPAIMSASLGDRVTMTCTASSSVSSSYLHWYQQKPGSSPKLWIYSTSNLASGVPARFSGSGSGTSYSLTISSMEAEDAATYYCHQFHRSLTFGSGTKLE |
Antigen
Chain: B
Mutation: NULL
| >2A0L_B|Chain A, B|Voltage-gated potassium channel|Aeropyrum pernix (56636) LGLSDLGGRVRNIGDVMEHPLVELGVSYAALLSVIVVVVEYTMQLSGEYLVRLYLVDLILVIILWADYAYRAYKSGDPAGYVKKTLYEIPALVPAGLLALIEGHLAGLGLFRLVRLLRFLRILLIISRGSKFLSAIADAADKIRFYHLFGAVMLTVLYGAFAIYIVEYPDPNSSIKSVFDALWWAVVTATTVGYGDVVPATPIGKVIGIAVMLTGISALTLLIGTVSNMFQKILVGEPEPS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: TYR33 PHE92 F: TYR27 ASN31 ASN32 TYR33 ALA34 TYR51 ASN53 TYR54 THR57 GLY99 TYR100 ASP101 TYR102 PHE103 B: GLU107 ALA111 GLY112 LEU113 GLY114 LEU115 PHE116 ARG117 VAL119 ARG120 ARG123 ARG126 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
