Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2078 |
| PDBID: | 6BIT |
| Chains: | JL_G |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.19 |
| Reference: | 10.1073/pnas.1710877114 |
| Antibody | |
| Antibody: | KWAR23 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tyrosine-protein phosphatase non-receptor type substrate 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: J
Mutation: NULL
| >6BIT_J|Chain A[auth I], D[auth J]|KWAR23 Fab heavy chain|Mus musculus (10090) EVQLQQSGAELVKPGASVKLSCTASGFNIKDYYIHWVQQRTEQGLEWIGRIDPEDGETKYAPKFQDKATITADTSSNTAYLHLSSLTSEDTAVYYCARWGAYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPR |
Light Chain: L
Mutation: NULL
| >6BIT_L|Chain B[auth K], E[auth L]|KWAR23 Fab light chain|Mus musculus (10090) EIVLTQSPAIMSASPGEKVTLTCASSSVSSSYLYWYQQKPGSSPKLWIYSTSNLASGVPARFSGSGSGTSYSLTISSMEAEDAASYFCHQWSSYPRTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRG |
Antigen
Chain: G
Mutation: NULL
| >6BIT_G|Chain C[auth G], F[auth H]|Tyrosine-protein phosphatase non-receptor type substrate 1|Homo sapiens (9606) EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| J: LYS30 ASP31 TYR32 TYR33 HIS35 ARG50 ASP52 GLU54 ASP55 GLU57 LYS59 ARG98 TRP99 GLY100 ALA101 TYR102 L: SER30 SER31 TYR32 TYR34 LEU46 TYR49 SER50 ASN53 ALA55 SER56 TRP91 TYR94 ARG96 G: VAL33 GLN37 PHE39 PRO44 ARG46 GLU47 LEU48 TYR50 ASN51 GLN52 LYS53 GLU54 GLY55 HIS56 PHE57 PRO58 ARG59 ASP65 LEU66 THR67 LYS96 GLY97 SER98 PRO99 ASP100 ASP101 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
