Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2055 |
| PDBID: | 6B9J |
| Chains: | HL_X |
| Organism: | Vaccinia virus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1074/jbc.M117.814541 |
| Antibody | |
| Antibody: | vv138 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | vaccinia virus D8 protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6B9J_H|Chain B[auth A], E[auth H]|Fab vv138 Heavy chain|Homo sapiens (9606) EVQLVQSGAEVKKDLASVKVSCKVSGYTFTDYYMHWVQQAPGKGLEWMGLVDPQEGETTYAEKFQGRVTITADTSTDTAYMELSSLRSEDTAVYYCAKESFGIPHFWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: L
Mutation: NULL
| >6B9J_L|Chain C[auth B], F[auth L]|Fab vv138 Light chain|Homo sapiens (9606) EIVLTQSPGTLSLSPGERATLSCRASQSVTSTYLAWHQQKPGQAPRLLIYSASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPPYTFGQGTKVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE |
Antigen
Chain: X
Mutation: NULL
| >6B9J_X|Chain A[auth X], D[auth Y]|IMV membrane protein|Vaccinia virus (10245) MPQQLSPINIETKKAISNARLKPLDIHYNESKPTTIQNTGKLVRINFKGGYISGGFLPNEYVLSSLHIYWGKEDDYGSNHLIDVYKYSGEINLVHWNKKKYSSYEEAKKHDDGLIIISIFLQVLDHKNVYFQKIVNQLDSIRSANTSAPFDSVFYLDNLLPSKLDYFTYLGTTINHSADAVWIIFPTPINIHSDQLSKFRTLLSLSNHEGKPHYITENYRNPYKLNDDTEVYYSGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 THR30 ASP31 TYR32 TYR33 HIS35 LEU50 ASP52 GLN54 GLU55 GLU57 THR59 ALA72 ASP73 THR74 SER75 GLU99 SER100 PHE101 GLY102 L: THR30 SER31 TYR33 TYR92 GLY93 PRO97 TYR98 X: THR34 THR35 GLN37 THR39 LYS41 ARG44 ASN46 SER64 SER65 HIS67 SER103 TYR104 GLU105 LYS108 ASP139 SER140 ARG142 SER143 ALA144 ASN145 THR146 SER147 ALA148 PRO149 ASN175 SER177 LEU203 SER204 LEU205 HIS213 TYR214 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
