Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2044 |
| PDBID: | 6B0E |
| Chains: | BA_E |
| Organism: | Homo sapiens, Plasmodium falciparum NF54 |
| Method: | XRD |
| Resolution (Å): | 3.30 |
| Reference: | 10.1038/s41467-017-01924-3 |
| Antibody | |
| Antibody: | 1260 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Pfs25 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >6B0E_B|Chain B|1260 antibody, heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCRASGYIFTSYGFSWVRQAPGQGLEWMGWISAYNGNTDYSQKLQGRVTMTTDTSTNTVYMELRTLQSDDTAVYYCARDRGDRLYYYYYYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKS |
Light Chain: A
Mutation: NULL
| >6B0E_A|Chain A|1260 antibody, light chain|Homo sapiens (9606) DVVMTQSPLSLPVTLGQPASISCRSSQSLVYSDGNTYLNWFQQGPGQSPRRLIYKVSNRDSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQATHWPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: N93Q/N146Q/N168Q
| >6B0E_E|Chain C[auth E]|25 kDa ookinete surface antigen|Plasmodium falciparum (5843) TGAKVTVDTVCKRGFLIQMSGHLECKCENDLVLVNEETCEEKVLKCDEKTVNKPCGDFSKCIKIDGNPVSYACKCNLGYDMVNNVCIPNECKQVTCGNGKCILDTSNPVKTGVCSCNIGKVPNVQDQNKCSKDGETKCSLKCLKEQETCKAVDGIYKCDCKDGFIIDQESSICTGTKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER27 ASP28 GLY29 ASN30 TYR49 VAL51 SER52 ASN53 SER56 SER67 B: GLN1 VAL2 ILE28 SER31 TYR32 TYR53 ASN54 ARG96 GLY97 ASP98 E: PHE13 ILE15 MET17 GLU22 LYS24 CYS25 GLU26 ASN27 ASP28 VAL30 ASP55 ASN81 ASN82 VAL83 THR93 GLY95 ASN96 GLY97 LYS98 ASN115 ILE116 LYS118 ASP131 LYS148 ALA149 VAL150 ASP151 GLY152 ILE153 LYS155 ASP157 ILE163 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
