Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2038 |
| PDBID: | 6AZ2 |
| Chains: | AF_B |
| Organism: | Homo sapiens, Saccharomyces cerevisiae S288C |
| Method: | XRD |
| Resolution (Å): | 2.48 |
| Reference: | 10.1016/j.jmb.2017.12.012 |
| Antibody | |
| Antibody: | 12E Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Histone chaperone ASF1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >6AZ2_A|Chain A, B[auth C]|Fab Heavy Chain|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFNVSYSSIHWVRQAPGKGLEWVAYIYPSSGYTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARSYSTKLAMDYWGQGTLVTVFNQIKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: F
Mutation: NULL
| >6AZ2_F|Chain E, F|Fab Light Chain|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSQWYPITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >6AZ2_B|Chain C[auth B], D|Histone chaperone ASF1|Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (559292) GSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSILVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEEELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER30 TYR31 SER32 TYR50 TYR52 SER54 SER55 TYR57 THR58 SER59 TYR100 SER101 LYS103 LEU104 F: GLN29 SER30 SER32 SER93 GLN94 TRP95 TYR96 B: ILE9 LYS10 VAL11 LEU12 ASN13 ASN14 PRO15 ALA16 LYS17 ASP20 PRO21 GLU23 GLU25 PHE28 ASP58 SER59 ILE60 LEU61 VAL62 GLY63 PRO64 PRO66 ASN70 LYS71 PHE72 VAL73 SER75 ASP77 VAL136 ALA141 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
