Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2037 |
| PDBID: | 6AYZ |
| Chains: | RC_M |
| Organism: | Homo sapiens, Saccharomyces cerevisiae S288C |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.1016/j.jmb.2017.12.012 |
| Antibody | |
| Antibody: | 12E Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Histone chaperone ASF1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: R
Mutation: NULL
| >6AYZ_R|Chain A[auth B], E[auth R]|Fab Heavy Chain|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFNVSYYSIHWVRQAPGKGLEWVASIYPYYGSTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARGYGWALDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: C
Mutation: NULL
| >6AYZ_C|Chain B[auth C], C[auth D]|Fab Light Chain|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQDGWSLITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: M
Mutation: NULL
| >6AYZ_M|Chain D[auth A], F[auth M]|Histone chaperone ASF1|Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (559292) GSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSILVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEEELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: GLN27 SER28 VAL29 SER30 SER31 ALA32 TYR49 ARG66 GLY68 ASP91 GLY92 TRP93 SER94 R: TYR31 TYR32 SER33 TYR52 PRO53 TYR54 TYR55 SER57 THR58 SER59 GLY99 TYR100 GLY101 TRP102 M: SER5 LEU6 LEU7 GLY8 ILE9 LYS10 GLU29 VAL45 SER47 SER48 ARG49 SER50 LEU51 ASP52 VAL92 THR93 VAL94 LEU96 ARG108 VAL109 GLY110 TYR111 TYR112 ASN114 ASN138 LEU140 ALA141 GLU142 LYS143 PRO144 ARG145 VAL146 THR147 ARG148 PHE149 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
