Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2020 |
| PDBID: | 6AL5 |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1002/prot.25485 |
| Antibody | |
| Antibody: | B43 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | B-lymphocyte antigen CD19 |
| Antigen mutation: | Yes |
| Durg Target: | P15391 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6AL5_H|Chain C[auth H]|B43 HEAVY CHAIN|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKASGYAFSSYWMNWVRQAPGQGLEWMGQIWPGDSDTNYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARRETTTVGRYYYAMDYWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHH |
Light Chain: L
Mutation: NULL
| >6AL5_L|Chain B[auth L]|B43 LIGHT CHAIN|Homo sapiens (9606) DIQLTQSPSFLSASVGDRVTITCKASQSVDYSGDSYLNWYQQKPGKAPKLLIYDASNLVSGVPSRFSGSGSGTEFTLTISSLQPEDFATYYCQQSTENPWTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: N118Q
| >6AL5_A|Chain A|B-lymphocyte antigen CD19|Homo sapiens (9606) EEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKQRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARGSHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR31 SER32 GLY33 ASP34 H: ALA28 SER30 SER31 TRP33 TRP52 GLY54 ASP55 ASP57 GLU74 ARG99 THR101 THR102 THR103 VAL104 GLY105 ARG106 TYR107 TYR109 A: LEU96 PRO102 SER103 LYS105 TRP107 GLU120 LYS155 TYR157 TRP159 ASP162 ARG163 PRO164 ILE166 GLU168 THR215 VAL217 HIS218 PRO219 LYS220 GLY221 PRO222 LYS223 SER224 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
