Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 20 |
| PDBID: | 1CZ8 |
| Chains: | HL_W |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.40 |
| Reference: | 10.1006/jmbi.1999.3192 |
| Antibody | |
| Antibody: | 12 Fab |
| Antibody mutation: | Yes |
| INN (Clinical Trial): | Faricimab(Approved); Ranibizumab(Approved); Tarcocimab(Phase-III); |
| Antigen | |
| Antigen: | Vascular endothelial growth factor A |
| Antigen mutation: | No |
| Durg Target: | P15692 |
Sequence information
Antibody
Heavy Chain: H
Mutation: T28D/N31H/H101Y/S105T/T231L
| >1CZ8_H|Chain D[auth H], F[auth Y]|HEAVY CHAIN OF NEUTRALIZING ANTIBODY|Mus musculus (10090) EVQLVESGGGLVQPGGSLRLSCAASGYDFTHYGMNWVRQAPGKGLEWVGWINTYTGEPTYAADFKRRFTFSLDTSKSTAYLQMNSLRAEDTAVYYCAKYPYYYGTSHWYFDVWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHL |
Light Chain: L
Mutation: M4L
| >1CZ8_L|Chain C[auth L], E[auth X]|LIGHT CHAIN OF NEUTRALIZING ANTIBODY|Mus musculus (10090) DIQLTQSPSSLSASVGDRVTITCSASQDISNYLNWYQQKPGKAPKVLIYFTSSLHSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYSTVPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: W
Mutation: NULL
| >1CZ8_W|Chain A[auth V], B[auth W]|Vascular endothelial growth factor A|Homo sapiens (9606) VVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR91 SER92 THR93 VAL94 TRP96 H: THR30 HIS31 TYR32 GLY33 TRP50 ASN52 THR53 TYR54 THR55 GLU57 THR59 TYR99 PRO100 TYR101 TYR102 TYR103 GLY104 THR105 SER106 HIS107 TRP108 W: TYR39 TYR45 LYS48 GLN79 ILE80 MET81 ARG82 ILE83 LYS84 PRO85 HIS86 GLN87 GLY88 GLN89 HIS90 ILE91 GLY92 GLU93 MET94 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
