Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1966 |
| PDBID: | 5XWD |
| Chains: | HD_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.89 |
| Reference: | 10.1371/journal.pone.0193158 |
| Antibody | |
| Antibody: | 059-152 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Epidermal growth factor receptor |
| Antigen mutation: | No |
| Durg Target: | P00533; Q504U8; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5XWD_H|Chain B[auth H]|VH chain of 059-152|Homo sapiens (9606) GSSGSSGQVQLQESGPGLVKPSETLSLTCTVSGGSISSSSYYWGWIRQPPGKGLEWIGSIYYSGSTYYNPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARLPMVTMSFDYWGQGTLVTVSSASTKG |
Light Chain: D
Mutation: NULL
| >5XWD_D|Chain C[auth D]|VL chain of 059-152|Homo sapiens (9606) GSSGSSGSYVLTQPPSVSVAPGKTARITCGGNNIGSKSVHWYQQKPGQAPVLVIYYDSDRPSGIPERFSGSNSGNTATLTISRVEAGDEADYYCQVWDSSSDHVVFGGGTKLTVLGQPKA |
Antigen
Chain: A
Mutation: NULL
| >5XWD_A|Chain A|Epidermal growth factor receptor|Homo sapiens (9606) MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIGSENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER30 SER32 SER33 TYR34 TYR35 TYR54 TYR55 SER56 GLY57 SER58 THR59 TYR60 ARG99 PRO101 MET102 VAL103 THR104 MET105 ASP108 D: GLY29 SER30 LYS31 SER32 TYR49 TYR50 ASP51 SER52 ASP53 TRP91 SER93 SER94 SER95 ASP96 A: LYS322 ASP323 SER324 LEU325 ASP344 HIS346 LEU348 PRO349 VAL350 ARG353 ASP355 PHE357 THR358 PHE380 LEU382 GLN384 LYS407 GLN408 HIS409 PHE412 ALA415 VAL417 SER418 ASN420 ILE438 SER440 GLY441 LYS443 ASN444 LYS465 ILE467 SER468 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
