Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1952 |
| PDBID: | 5XAJ |
| Chains: | FD_H |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1128/JVI.00406-17 |
| Antibody | |
| Antibody: | anti-idiotype E1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HM14c10 Fab heavy chain |
| Antigen mutation: | No |
| Durg Target: | Q6PYX1; P01857; P01859; P01860; P01861; |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >5XAJ_F|Chain F|Fab E1 heavy chain|Homo sapiens (9606) EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGGIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCAVGLPVDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: D
Mutation: NULL
| >5XAJ_D|Chain C, D|Fab E1 light chain|Homo sapiens (9606) LSYELTQPPSVSVAPGQTARMTCGGNNIGTKSVHWYQQKPGQAPVLVVYNDRDRPSGIPERFSGSNSGNTATLTISRVEDGDEADYFCQVWDNYSDHVIFGGGTKLTVLGAPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQVTHEGSTVEKTVAPTEC |
Antigen
Chain: H
Mutation: NULL
| >5XAJ_H|Chain A, G[auth H]|FabHM14c10 heavy chain|Homo sapiens (9606) EVQLVESGGGVVQPGRSLRLSCAASGFSFSSYGMHWVRQAPGKGLEWVAVIWYDGSKTYYGDSVKGRFTISKDNSKKMVNLQMDSLGVEDTAFYYCARGIAGGWAFWGIDLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCAA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: HIS34 LEU46 TYR49 ASN50 ARG52 ASP53 PRO55 SER56 TRP91 F: GLU1 VAL2 THR28 SER31 TYR32 ALA33 ILE52 ILE54 PHE55 GLY99 LEU100 PRO101 ASP103 TYR104 H: SER31 TRP52 TYR53 ASP54 GLY55 SER56 LYS57 THR58 TYR59 LYS65 GLY66 ALA101 GLY102 GLY103 TRP104 TRP107 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
