Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1951 |
| PDBID: | 5XAJ |
| Chains: | EC_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1128/JVI.00406-17 |
| Antibody | |
| Antibody: | anti-idiotype E1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HM14c10 Fab light chain |
| Antigen mutation: | No |
| Durg Target: | P01834 |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >5XAJ_E|Chain E|Fab E1 heavy chain|Homo sapiens (9606) EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGGIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCAVGLPVDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: C
Mutation: NULL
| >5XAJ_C|Chain C, D|Fab E1 light chain|Homo sapiens (9606) LSYELTQPPSVSVAPGQTARMTCGGNNIGTKSVHWYQQKPGQAPVLVVYNDRDRPSGIPERFSGSNSGNTATLTISRVEDGDEADYFCQVWDNYSDHVIFGGGTKLTVLGAPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQVTHEGSTVEKTVAPTEC |
Antigen
Chain: B
Mutation: NULL
| >5XAJ_B|Chain B, H[auth L]|FabHM14c10 light chain|Homo sapiens (9606) DIVMTQSPGTLSLSPGERATLSCRASQNVYSYLGWYQHKPGRSPRLLIFGVTSRATGVPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYAGSAYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: SER32 ASN50 TRP91 ASN93 ASP96 E: ALA33 ILE52 PHE55 B: GLN27 ASN28 TYR30 SER31 TYR32 GLY50 SER94 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
