Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1940 |
| PDBID: | 5X2P |
| Chains: | HL_A |
| Organism: | Oryzias latipes, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.61 |
| Reference: | 10.1038/ncomms15530 |
| Antibody | |
| Antibody: | Fab16A Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Taste receptor type 1 member 2a |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5X2P_H|Chain E[auth H], G[auth J]|Fab16A Heavy chain|Mus musculus (10090) EVQLQQSGPELVKPGASMKISCKASGYSFTGYSMNWVKQSHGKNLEWIGLINPYNGDTTYKQKFKGKATLTVDRSSSTAYMELLRLTSEDSAVYYCARSGRGAPTTTTAWFTYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRD |
Light Chain: L
Mutation: NULL
| >5X2P_L|Chain F[auth K], H[auth L]|Fab16A Light chain|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCRASESVDSYGNSFMHWYQQKPGQPPILLISRASNLESGIPARFSGSGSRTDFTLTINPVEADDFATYYCQQTNEDPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: A
Mutation: NULL
| >5X2P_A|Chain A, C|Taste receptor, type 1, member 2a|Oryzias latipes (8090) QSTDQTSEFHLRGDYLIGGLFNIHYVAAANFQRPQAIDCSSKLFILPNYRRFQMMRFSVEEINNSSSLLPNVSLGYQMFDHCSDIHSFPGIFKLLSVNDLIRPWEDASTGLPNAIGVVGPFTSTHALSIAPIFMTNLFPMVSYGCSGSVFSKENLYPSFLRTVHSNKDVINAIVGIILNFNWRWVAFLYSDDDFGKDGLEQFKNKIEDSEICLAFYKAINVNTDYLQVFKQIEEQNIKVIVVFAPKVYAEAVVESAVQLNVTNKVWIADDGWSLNKKLPSMNGIQNIGTVLGVAQPVVTIPGFTDFIYSAISQTDGGDTEQKMFCNQKCNCSNLSVKSLLNADPSFSFPVYAAVYAIAHALHNTLRCGSDRCPKNITVHPHMILEELKKSNFTLLNQTVQFDENGDPKFGSLSVVFWNSSGNAEEVGSYHFQSSIHLSINKTKIKWFTNGEVPTSSGIEGR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR27 SER28 THR30 GLY31 TYR32 SER33 ASN52 TYR54 ASN55 ASP57 ARG74 SER99 GLY100 ARG101 GLY102 ALA103 PRO104 THR105 THR106 THR107 ALA109 L: TYR32 GLY33 ASN34 ARG54 A: GLY194 LEU197 ASN198 ASN200 TRP201 ARG202 TRP203 ILE225 GLU226 ASP227 SER228 GLU229 ILE230 CYS231 LEU232 ASN255 LYS257 SER453 ILE454 HIS455 LEU456 LYS460 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
