Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1930 |
| PDBID: | 5WTH |
| Chains: | ED_B |
| Organism: | Hepatovirus A, Mus musculus |
| Method: | EM |
| Resolution (Å): | 4.20 |
| Reference: | 10.1073/pnas.1616502114 |
| Antibody | |
| Antibody: | R10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatitis A virus VP2 |
| Antigen mutation: | No |
| Durg Target: | P06441; P08617; P13901; |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >5WTH_E|Chain E|FAB Heavy Chain|Mus musculus (10090) EVKLVESGGGSVKPGGSLKLSCAASGFSFSTYGMSWVRQTPEKRLEWVATISGGGGYTYYPDSVKGRFTISRDNARNILYLQMSSLRSGDTAMYYCARRVTTVAEYYFDYWGQGTTLTVSSPKTTPPSVYPLAPASASTAASMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPR |
Light Chain: D
Mutation: NULL
| >5WTH_D|Chain D|FAB Light Chain|Mus musculus (10090) DIVLTQSPAIMSASPGEKVTMTCSASSSVSYIHWYQQKSGTSPKRWIYDTSRLAFGVPGRFSGSGSGTSYSLTISSMEAEDAATYYCQQWSSNYTFGGGTNLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: NULL
| >5WTH_B|Chain B|VP2|Hepatitis A virus (12092) DIEEEQMIQSVDRTAVTGASYFTSVDQSSVHTAEVGSHQIEPLKTSVDKPGSKKTQGEKFFLIHSARWLTTHALFHEVAKLDVVKLLYNEQFAVQGLLRYHTYARFGIEIQVQINPTPFQQGGLICAMVPGDQSYGSIASLTVYPHGLLNCNINNVVRIKVPFIYTRGAYHFKDPQYPVWELTIRVWSELNIGTGTSAYTSLNVLARFTDLELHGLTPLSTQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: TYR31 TYR48 SER51 ARG52 LEU53 PHE55 GLY59 ARG60 PHE61 SER62 E: VAL103 ALA104 B: LEU62 HIS64 SER65 ALA66 ARG67 LEU69 THR71 GLN91 ALA198 TYR199 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
