Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1924 |
| PDBID: | 5WI9 |
| Chains: | FE_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1074/jbc.RA118.004343 |
| Antibody | |
| Antibody: | 39F7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Beta-klotho |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >5WI9_F|Chain D[auth F], F[auth H]|39F7 Fab heavy chain|Homo sapiens (9606) QVQLVESGGGVVQPGRSLRLSCAASGFTFSNYGIHWVRQAPGKGLEWVAVIWYDGSIKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARDRAAAGLHYYYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: E
Mutation: NULL
| >5WI9_E|Chain C[auth E], E[auth L]|39F7 Fab light chain|Homo sapiens (9606) EIVLTQSPGTLSLSPGERATLSCRASQSVSSTYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQSGSSPLTFGGGTEVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >5WI9_B|Chain A, B|Beta-klotho|Homo sapiens (9606) GFSGDGRAIWSKNPNFTPVNESQLFLYDTFPKNFFWGIGTGALQVEGSWKKDGKGPSIWDHFIHTHLKNVSSTNGSSDSYIFLEKDLSALDFIGVSFYQFSISWPRLFPDGIVTVANAKGLQYYSTLLDALVLRNIEPIVTLYHWDLPLALQEKYGGWKNDTIIDIFNDYATYCFQMFGDRVKYWITIHNPYLVAWHGYGTGMHAPGEKGNLAAVYTVGHNLIKAHSKVWHNYNTHFRPHQKGWLSITLGSHWIEPNRSENTMDIFKCQQSMVSVLGWFANPIHGDGDYPEGMRKKLFSVLPIFSEAEKHEMRGTADFFAFSFGPNNFKPLNTMAKMGQNVSLNLREALNWIKLEYNNPRILIAENGWFTDSRVKTEDTTAIYMMKNFLSQVLQAIRLDEIRVFGYTAWSLLDGFEWQDAYTIRRGLFYVDFNSKQKERKPKSSAHYYKQIIRENGFENLYFQGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: GLU1 ILE2 ARG24 GLN27 SER28 VAL29 SER30 SER31 THR32 TYR33 TYR50 GLY51 SER53 SER54 GLY67 SER68 GLY69 THR70 ASP71 SER92 GLY93 SER94 SER95 LEU97 F: SER30 ASN31 TYR53 ASP54 SER56 ILE57 LYS58 TYR59 TYR60 ALA103 GLY104 LEU105 HIS106 TYR107 TYR108 TYR109 B: TYR78 PRO82 LYS83 ASN84 PHE86 GLU188 LYS234 TYR235 TRP281 HIS282 ASN285 ARG289 PRO290 LYS293 GLY294 TRP295 HIS335 GLY336 HIS361 ARG364 GLY365 ALA367 ASP368 PHE369 ASN408 ASN409 PRO410 ARG411 ILE447 ARG448 GLU451 ILE452 ARG453 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
