Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1880 |
| PDBID: | 5W23 |
| Chains: | JN_A |
| Organism: | Human respiratory syncytial virus A2, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.40 |
| Reference: | 10.1038/s41467-017-01858-w |
| Antibody | |
| Antibody: | 5C4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Respiratory syncytial virus (RSV) Fusion glycoprotein F0 |
| Antigen mutation: | No |
| Durg Target: | P03420; P11209; |
Sequence information
Antibody
Heavy Chain: J
Mutation: NULL
| >5W23_J|Chain D[auth H], F[auth I], H[auth J]|5C4 Fab heavy chain|Mus musculus (10090) MRPTWAWWLFLVLLLALWAPARGEVQLQQSGAELVKPGASVKLSCTASGFNIKDTFFHWVKQRPEQGLEWIGRIDPADGHTKYDPKFQGKATITADTSSNTAFLQLSSLTSVDTAVYYCATTITAVVPTPYNAMDYWGQGTSVTVSSASTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIEPRVP |
Light Chain: N
Mutation: NULL
| >5W23_N|Chain E[auth L], G[auth M], I[auth N]|5C4 Fab light chain|Mus musculus (10090) MRPTWAWWLFLVLLLALWAPARGDIVLTQSPASLAVSLGQRTTISCRASESVDSFDNSFIHWYQQKPGQPPKLLIFLASSLESGVPARFSGSGSRTDFTLTIDPVEADDAATYYCQQSNEDPFTFGSGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >5W23_A|Chain A, B, C|Fusion glycoprotein F0|Human respiratory syncytial virus A (11259) MELLILKANAITTILTAVTFCFASGQNITEEFYQSTCSAVSKGYLSALRTGWYTSVITIELSNIKENKCNGTDAKVKLIKQELDKYKNAVTELQLLMQSTPATNNRARRELPRFMNYTLNNAKKTNVTLSKKRKRRFLGFLLGVGSAIASGVAVSKVLHLEGEVNKIKSALLSTNKAVVSLSNGVSVLTSKVLDLKNYIDKQLLPIVNKQSCSISNIETVIEFQQKNNRLLEITREFSVNAGVTTPVSTYMLTNSELLSLINDMPITNDQKKLMSNNVQIVRQQSYSIMSIIKEEVLAYVVQLPLYGVIDTPCWKLHTSPLCTTNTKEGSNICLTRTDRGWYCDNAGSVSFFPQAETCKVQSNRVFCDTMNSLTLPSEVNLCNVDIFNPKYDCKIMTSKTDVSSSVITSLGAIVSCYGKTKCTASNKNRGIIKTFSNGCDYVSNKGVDTVSVGNTLYYVNKQEGKSLYVKGEPIINFYDPLVFPSDEFDASISQVNEKINQSLAFIRKSDELLSAIGGYIPEAPRDGQAYVRKDGEWVLLSTFLGGLVPRGSHHHHHHSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| J: PHE27 LYS30 ASP31 THR32 PHE33 ALA53 ASP54 HIS56 THR95 ILE96 ALA98 VAL99 N: SER27 PHE28 ASP29 ASN30 PHE32 PHE49 LEU50 ALA51 SER52 SER53 ASN92 A: ASN63 ILE64 LYS65 GLU66 ASN67 LYS68 CYS69 LYS196 ASN197 TYR198 ASP200 LYS201 GLN202 LEU204 PRO205 ILE206 VAL207 ASN208 LYS209 SER211 CYS212 GLU294 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
