Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1865 |
| PDBID: | 5W0K |
| Chains: | EF_C |
| Organism: | Human herpesvirus 4 strain B95-8, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.1073/pnas.1704661114 |
| Antibody | |
| Antibody: | CL40 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Herpesvirus envelope glycoprotein H |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >5W0K_E|Chain C[auth E], H|CL40 IgG heavy chain|Mus musculus (10090) EVKLQQSGAELVMPGASVKMSCKASGYTFTDYWMHWVKQRPGQGLEWIGAIDTSDSYTSYNQKFKGKATLTVDESSSTAYMQLSSLTSEDSAVYYCARGLLPFDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRD |
Light Chain: F
Mutation: NULL
| >5W0K_F|Chain D[auth F], I[auth L]|CL40 IgG light chain|Mus musculus (10090) DIVMTQSQKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKALIYSASYRYSGVPDRFTGSGSGTDFTLTISNVQSEDLAKYFCQQYNSYPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: C
Mutation: NULL
| >5W0K_C|Chain A, F[auth C]|Envelope glycoprotein H|Human herpesvirus 4 (strain B95-8) (10377) LSEVKLHLDIEGHASHYTIPWTELMAKVPGLSPEALWREANVTEDLASMLNRYKLIYKTSGTLGIALAEPVDIPAVSEGSMQVDASKVHPGVISGLNSPACMLSAPLEKQLFYYIGTMLPNTRPHSYVFYQLRCHLSYVALSINGDKFQYTGAMTSKFLMGTYKRVTEKGDEHVLSLVFGKTKDLPDLRGPFSYPSLTSAQSGDYSLVIVTTFVHYANFHNYFVPNLKDMFSRAVTMTAASYARYVLQKLVLLEMKGGCREPELDTETLTTMFEVSVAFFKVGHAVGETGNGCVDLRWLAKSFFELTVLKDIIGICYGATVKGMQSYGLERLAAMLMATVKMEELGHLTTEKQEYALRLATVGYPKAGVYSGLIGGATSVLLSAYNRHPLFQPLHTVMRETLFIGSHVVLRELRLNVTTQGPNLALYQLLSTALCSALEIGEVLRGLALGTESGLFSPCYLSLRFDLTRDKLLSMAPQEATLDQAAVSNAVDGFLGRLSLEREDRDAWHLPAYKCVDRLDKVLMIIPLINVTFIISSDREVRGSALYEASTTYLSSSLFLSPVIMNKCSQGAVAGEPRQIPKIQNFTRTQKSCIFCGFALLSYDEKEGLETTTYITSQEVQNSILSSNYFDFDNLHVHYLLLTTNGTVMEIAGLYEERAH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: ASP31 TYR32 TRP33 ASP52 SER54 ASP55 TYR57 SER59 TYR60 ASN61 GLU74 GLY99 LEU100 LEU101 F: ASP1 VAL3 THR5 LYS24 ALA25 SER26 GLN27 ASN28 GLY30 THR31 ASN32 SER50 TYR53 SER67 TYR91 ASN92 SER93 TYR94 PRO95 TYR96 C: LEU82 ASN163 LYS166 ARG184 THR186 GLU187 LYS188 HIS192 ALA236 ASN237 HIS239 ASN240 TYR241 VAL243 ASN245 ASP248 LYS275 GLU282 LEU283 ASP284 THR285 GLU286 ALA467 THR500 ASP502 GLN503 ALA504 ALA505 ASN508 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
