Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1861 |
| PDBID: | 5W08 |
| Chains: | OP_E |
| Organism: | Influenza A virus (A/Texas/50/2012(H3N2)), Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1016/j.immuni.2017.12.009 |
| Antibody | |
| Antibody: | K03.12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H3N2 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: O
Mutation: NULL
| >5W08_O|Chain G, I, K, M, O, Q|K03.12 antibody heavy chain|Homo sapiens (9606) EVQLVQSGAEVKKPGASVKVSCKTSGYTFTAYYLHWVRQAPGQGFEWMAWINPNTGDTNYAQKFQGRVTLSRDTSITTAYMELTRLRSDDTAVYYCAKDLTLMYVFDSGWARGAHDYYGMDVWGQGTTVAVSGASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKHHHHHH |
Light Chain: P
Mutation: NULL
| >5W08_P|Chain H, J, L, N, P, R|K03.12 antibody light chain|Homo sapiens (9606) PSALTQPASVSGSPGQSVTISCTGTNSDVGTFDLVSWYQQYPGKAPKLIIYEGSRRPSGVSDRFSGSKSGNTASLTISGLQAEDEADYYCSSYAGSVVFGGGTKLTVLGQPKGAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: E
Mutation: NULL
| >5W08_E|Chain A, B, C, D, E, F|Hemagglutinin HA1|Influenza A virus (A/Texas/50/2012(H3N2)) (1321009) TNATELVQNSSIGEICDSPHQILDGENCTLIDALLGDPQCDGFQNKKWDLFVERSKAYSNCYPYDVPDYASLRSLVASSGTLEFNNESFNWNGVTQNGTSSACIRRSNNSFFSRLNWLTHLNFKYPALNVTMPNNEQFDKLYIWGVHHPVTDKDQIFLYAQPSGRITVSTKRSQQAVIPNIGFRPRIRNIPSRISIYWTIVKPGDILLINSTGNLIAPRGYFKIRSGKSSIMRSDAPIGKCKSECITPNGSIPNDKPFQNVNRITYGACPRYVKQSTLKLATGGALEVLFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| O: THR28 TYR32 LEU100 THR101 LEU102 TYR104 VAL105 ASP107 SER108 GLY109 TRP110 ALA111 ARG112 GLY113 ALA114 TYR118 P: TYR51 GLU52 ARG55 E: TYR98 THR131 GLN132 ASN133 GLY134 THR135 SER136 SER137 ASN145 TRP153 THR155 HIS156 LEU157 ASN158 PHE159 LYS160 HIS183 VAL186 THR187 LYS189 ASP190 PHE193 LEU194 ARG222 ASN225 ILE226 PRO227 SER228 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
