Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1843 |
| PDBID: | 5VPL |
| Chains: | DC_A |
| Organism: | Dermatophagoides farinae, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 1.90 |
| Reference: | 10.1074/jbc.M111.311159 |
| Antibody | |
| Antibody: | 4C1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Der f 1 variant |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >5VPL_D|Chain C[auth D]|4C1 - HEAVY CHAIN|Mus musculus (10090) EVQLQESGPGLVKPSQSLSLTCTVTGYSITSDYAWNWIRQFPGNKLEWMGYISYSGTTSYNPSLKSRISITRDTSKNQFFLQLNSVTTEDTATYYCGRTGVYRYPERAPYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTP |
Light Chain: C
Mutation: NULL
| >5VPL_C|Chain B[auth C]|4C1 - LIGHT CHAIN|Mus musculus (10090) QIVMTQSPFSMYATLGERVTITCKASQDIYSYLSWLQQKPGKSLKTLIYRANRLITGVPSRFSGSGSGQDYSLTISSLEYEDMGIYYCLQYDEFPYTFGGGTKLEMKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: A
Mutation: NULL
| >5VPL_A|Chain A|Der f 1 variant|Dermatophagoides farinae (6954) TSACRINSVNVPSELDLRSLRTVTPIRMQGGCGSCWAFSGVAATESAYLAYRNTSLDLSEQELVDCASQHGCHGDTIPRGIEYIQQNGVVEERSYPYVAREQQCRRPNSQHYGISNYCQIYPPDVKQIREALTQTHTAIAVIIGIKDLRAFQHYDGRTIIQHDNGYQPNYHAVNIVGYGSTQGVDYWIVRNSWDTTWGDSGYGYFQAGNNLMMIEQYPYVVIM |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: TYR30 TYR32 ARG50 ASN52 ARG53 ASP92 GLU93 D: THR30 SER31 ASP32 TYR54 SER55 ARG72 VAL101 TYR102 ARG103 TYR104 PRO105 GLU106 ARG107 A: GLU14 ASP16 ARG18 SER19 ARG21 ASP155 GLY156 ARG157 THR158 ILE159 THR181 GLN182 TYR186 ILE188 ASP199 SER200 TYR202 TYR204 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
