Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1832 |
| PDBID: | 5VLP |
| Chains: | HL_A |
| Organism: | Homo sapiens, Mus musculus, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1038/nsmb.3453 |
| Antibody | |
| Antibody: | 7G7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Proprotein convertase subtilisin/kexin type 9 |
| Antigen mutation: | Yes |
| Durg Target: | Q8NBP7 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5VLP_H|Chain B[auth H]|Fab7G7 heavy chain|Mus musculus (10090) QVQLKQSGAELVRPGASVKLSCKASGYIFTDYYINWLKKRPGQGLEWIARIYPGSGHTYYNENFKDKATLTAEKSSSNVYMQLSSLTSEDSAVYFCARENFYGSSYVDWYFDVWGTGTTVTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEP |
Light Chain: L
Mutation: NULL
| >5VLP_L|Chain C[auth L]|Fab7G7 light chain|Mus musculus (10090) DIVMTQSQKFMSTSGGDRVSITCKTSQNVGTAVAWFQQKPGQSPKLLIYSASNRYTGVSDRFTGSGSGTEFIFTISYAQSEDLADYFCHQYSSYPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: V474I/G670E
| >5VLP_A|Chain A|Proprotein convertase subtilisin/kexin type 9|Homo sapiens (9606) MGTVSSRRSWWPLPLLLLLLLLLGPAGARAQEDEDGDYEELVLALRSEEDGLAEAPEHGTTATFHRCAKDPWRLPGTYVVVLKEETHLSQSERTARRLQAQAARRGYLTKILHVFHGLLPGFLVKMSGDLLELALKLPHVDYIEEDSSVFAQSIPWNLERITPPRYRADEYQPPDGGSLVEVYLLDTSIQSDHREIEGRVMVTDFENVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGASMRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSDCSTCFVSQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHGAGWQLFCRTVWSAHSGPTRMATAIARCAPDEELLSCSSFSRSGKRRGERMEAQGGKLVCRAHNAFGGEGVYAIARCCLLPQANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVTVACEEGWTLTGCSALPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSEEAVTAVAICCRSRHLAQASQELQHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ASP31 TYR33 ARG50 TYR52 SER55 HIS57 THR58 TYR59 LYS65 PHE101 TYR102 GLY103 SER104 SER105 TYR106 VAL107 ASP108 L: ILE2 GLN27 ASN28 VAL29 GLY30 THR31 ALA32 SER92 SER93 TYR94 A: ARG499 SER535 VAL536 HIS537 THR538 ALA539 PRO540 PRO541 ALA542 GLU543 ALA544 MET546 GLY547 THR548 ARG549 VAL550 HIS551 HIS553 GLN554 GLN555 HIS557 GLU567 VAL568 THR573 LYS575 GLU593 SER595 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
