Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 182 |
| PDBID: | 1WEJ |
| Chains: | HL_F |
| Organism: | Mus musculus, Equus caballus |
| Method: | XRD |
| Resolution (Å): | 1.80 |
| Reference: | 10.1006/jmbi.1998.1942 |
| Antibody | |
| Antibody: | E8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | CYTOCHROME C |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >1WEJ_H|Chain B[auth H]|E8 ANTIBODY|Mus musculus (10090) EVQLQQSGAELVKPGASVKLSCTASGFNIKDTYMHWVKQRPEKGLEWIGRIDPASGNTKYDPKFQDKATITADTSSNTAYLQLSSLTSEDTAVYYCAGYDYGNFDYWGQGTTLTVSSAETTPPSVYPLAPGTAALKSSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLTSSVTVPSSTWPSQTVTCNVAHPASSTKVDKKIVPRNCGGDC |
Light Chain: L
Mutation: NULL
| >1WEJ_L|Chain A[auth L]|E8 ANTIBODY|Mus musculus (10090) DIQMTQSPASLSASVGETVTITCRASGNIHNYLAWYQQKQGKSPQLLVYNAKTLADGVPSRFSGSGSGTQYSLKINSLQPEDFGSYYCQHFWSTPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: F
Mutation: NULL
| >1WEJ_F|Chain C[auth F]|CYTOCHROME C|Equus caballus (9796) XGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFTYTDANKNKGITWKEETLMEYLENPKKYIPGTKMIFAGIKKKTEREDLIAYLKKATNE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASN28 HIS30 ASN31 TYR32 ASN50 LYS52 GLY68 PHE91 TRP92 H: LYS30 ASP31 THR32 TYR33 ARG50 ASP52 SER55 ASN57 ASP100 TYR101 GLY102 F: VAL3 LYS22 GLY34 PHE36 GLY37 GLY56 THR58 LYS60 GLU61 GLU62 ALA96 LYS99 LYS100 ASN103 GLU104 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
