Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1817 |
| PDBID: | 5VJO |
| Chains: | AB_E |
| Organism: | Mus musculus, Anas platyrhynchos |
| Method: | XRD |
| Resolution (Å): | 2.43 |
| Reference: | 10.1126/science.aao3859 |
| Antibody | |
| Antibody: | HyHEL10 Fab |
| Antibody mutation: | Yes |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Lysozyme isoform I (DEL-I) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: I29F
| >5VJO_A|Chain A, C|HyHEL10 heavy chain Fab fragment carrying I29F mutation.|Mus musculus (10090) DVQLQESGPSLVKPSQTLSLTCSVTGDSFTSDYWSWIRKFPGNRLEYMGYVSYSGSTYYNPSLKSRISITRDTSKNQYYLDLNSVTTEDTATYYCANWDGDYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPR |
Light Chain: B
Mutation: NULL
| >5VJO_B|Chain B, D|HyHEL10 light chain Fab fragment|Mus musculus (10090) DIVLTQSPATLSVTPGNSVSLSCRASQSIGNNLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGTDFTLSINSVETEDFGMYFCQQSNSWPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNR |
Antigen
Chain: E
Mutation: NULL
| >5VJO_E|Chain E, F|lysozyme isoform I (DEL-I)|Anas platyrhynchos (8839) KVYSRCELAAAMKRLGLDNYRGYSLGNWVCAANYESSFNTQATNRNTDGSTDYGILQINSRWWCDDGKTPGSKNACGIPCSVLLRSDITEAVRCAKRIVSDGNGMNAWVAWRNRCRGTDVSKWIRGCRL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ASP27 THR30 SER31 ASP32 TYR33 TYR50 SER52 TYR53 SER54 SER56 TYR58 TRP98 ASP99 B: GLN27 SER28 GLY30 ASN31 ASN32 LYS49 TYR50 GLN53 SER67 SER91 ASN92 SER93 TRP94 TYR96 E: LYS13 ARG14 LEU15 GLY16 ASP18 ASN19 TYR20 ARG21 TRP62 TRP63 LYS73 ALA75 THR89 GLU90 ARG93 LYS96 ARG97 VAL99 SER100 ASP101 GLY102 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
