Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1782 |
| PDBID: | 5USL |
| Chains: | HL_X |
| Organism: | Vaccinia virus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1074/jbc.M117.814541 |
| Antibody | |
| Antibody: | vv304 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Vaccinia virus (VACV) envelope protein D8 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5USL_H|Chain C[auth B], E[auth H]|Fab vv304 Heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCVVSGFTFSNYWMSWVRQAPGKGLEWVANIKQDGSKKYYVDSVTGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCATLNLELAVDAISEALKWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: L
Mutation: NULL
| >5USL_L|Chain D[auth C], F[auth L]|Fab vv304 Light chain|Homo sapiens (9606) SYELTQPPSVSVSPGQTARITCSGDALPKQFAYWYQQKPGQAPVVMIYKDSERPSGIPERFSGSSSGTTVTSTISGVQAEDEADYYCQSVDNSGTYEVFGGGTQLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNSYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTMAHA |
Antigen
Chain: X
Mutation: NULL
| >5USL_X|Chain A, B[auth X]|IMV membrane protein|Vaccinia virus (10245) MPQQLSPINIETKKAISNARLKPLDIHYNESKPTTIQNTGKLVRINFKGGYISGGFLPNEYVLSSLHIYWGKEDDYGSNHLIDVYKYSGEINLVHWNKKKYSSYEEAKKHDDGLIIISIFLQVLDHKNVYFQKIVNQLDSIRSANTSAPFDSVFYLDNLLPSKLDYFTYLGTTINHSADAVWIIFPTPINIHSDQLSKFRTLLSLSNHEGKPHYITENYRNPYKLNDDTEVYYSGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER30 ASN31 TRP33 LYS52 GLN53 ASP54 ASN100 LEU101 GLU102 LEU103 ALA104 VAL105 ASP106 ALA107 ILE108 SER109 GLU110 L: LEU27 PRO28 LYS29 GLN30 PHE31 TYR48 LYS49 ASP50 SER51 GLU52 ARG53 SER65 SER93 GLY94 THR95 X: GLU30 LYS32 PRO33 THR34 THR35 GLN37 ASN38 THR39 ARG44 ASN46 PHE47 LYS48 GLY49 SER64 TRP96 LYS98 SER102 SER103 TYR104 GLU105 SER140 SER143 ASN145 THR146 SER147 ALA148 PRO149 PHE150 ASP151 SER152 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
