Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 178 |
| PDBID: | 1V7N |
| Chains: | IM_X |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.30 |
| Reference: | 10.1073/pnas.0308530100 |
| Antibody | |
| Antibody: | TN1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human Thrombopoietin Functional Domain |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >1V7N_I|Chain B[auth H], D[auth I], F[auth J], H[auth K]|Monoclonal TN1 Fab Heavy Chain|Mus musculus (10090) EVKLEESGGGLVQPGGSMKLSCAASGFTFSDAWMDWVRQSPEKGLEWVAEIRSKVNNHAIHYAESVKGRFTVSRDDSKSSVYLQMNSLRAEDTGIYYCSGWSFLYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRD |
Light Chain: M
Mutation: NULL
| >1V7N_M|Chain A[auth L], C[auth M], E[auth N], G[auth O]|Monoclonal TN1 Fab Light Chain|Mus musculus (10090) QVVLTQSPGIMSASPGEKVTITCSASSSVSYMYWFQQKPGTSPKLWIYSTSNLASGVPARFRGSGSGTSYSLTISRMEAEDAATYYCQQRSGYPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: X
Mutation: Q115R
| >1V7N_X|Chain I[auth V], J[auth X], K[auth Y], L[auth Z]|Thrombopoietin|Homo sapiens (9606) SPAPPACDLRVLSKLLRDSHVLHSRLSQCPEVHPLPTPVLLPAVDFSLGEWKTQMEETKAQDILGAVTLLLEGVMAARGQLGPTCLSSLLGQLSGQVRLLLGALQSLLGTQLPPRGRTTAHKDPNAIFLSFQHLLRGKVRFLMLVGGSTLCVRRAPPTTAVPS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: SER30 TYR31 TYR33 LEU45 TYR48 SER49 ASN52 LEU53 ALA54 ARG90 TYR93 ARG95 I: THR28 ASP31 TRP33 ARG52 VAL55 ASN56 TRP101 SER102 LEU104 TYR105 X: GLU57 THR58 GLN61 THR68 LEU71 GLU72 MET75 ARG98 LEU99 LEU101 GLY102 GLN105 SER106 GLY109 THR110 GLN111 LEU112 PRO113 PRO114 ARG115 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
