Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1743 |
| PDBID: | 5UK2 |
| Chains: | I_E |
| Organism: | Influenza A virus (A/Solomon Islands/3/2006(H1N1)), Homo sapiens |
| Method: | EM |
| Resolution (Å): | 4.80 |
| Reference: | 10.1016/j.jmb.2017.05.011 |
| Antibody | |
| Antibody: | K1915 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H1N1 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: I
Mutation: NULL
| >5UK2_I|Chain G, H, I|scFv|Homo sapiens (9606) EIVLTQSPGTLSLSPGERATLSCRASQSISGYYLTWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGISPVITFGGGTNVEIKGGGGGSGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCAASGFTFNIYDMHWVRQAPGKGLEWVSGLTTGGDTSYSGSVRGRFSISRENAKNSLYLQMNNLRAGDTAAYFCVRGVREVGATGGDPFYYAMAVWGQGTTVTVSSASGSSGSGHHHHHH |
Antigen
Chain: E
Mutation: NULL
| >5UK2_E|Chain A, C, E|Hemagglutinin HA1|Influenza A virus (A/Solomon Islands/3/2006(H1N1)) (464623) EDTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCLLKGIAPLQLGNCSVAGWILGNPECELLISRESWSYIVEKPNPENGTCYPGHFADYEELREQLSSVSSFERFEIFPKESSWPNHTTTGVSASCSHNGESSFYKNLLWLTGKNGLYPNLSKSYANNKEKEVLVLWGVHHPPNIGDQRALYHTENAYVSVVSSHYSRKFTPEIAKRPKVRDREGRINYYWTLLEPGDTIIFEANGNLIAPRYAFALSRGFGSGIINSNAPMDECDAKCQTPQGAINSSLPFQNVHPVTIGECPKYVRSAKLRMVTGLRNIPS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: SER30 TYR32 TYR33 TYR92 ILE94 ASN155 ILE156 LEU176 THR178 GLY179 GLY180 ASP181 ASN198 ARG225 GLU226 VAL227 GLY228 ALA229 THR230 GLY231 PRO234 PHE235 TYR237 E: TYR95 THR131 THR133 GLY134 VAL135 SER136 ALA137 GLY143 GLU144 SER145 SER146 TRP153 THR155 GLY156 LYS157 ASN158 GLY159 LEU160 HIS183 PRO186 ASN187 GLY189 ASP190 ALA193 LEU194 HIS196 LYS222 ASP225 ARG226 GLU227 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
