Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1731 |
| PDBID: | 5UHY |
| Chains: | JI_C |
| Organism: | Homo sapiens, Zika virus |
| Method: | EM |
| Resolution (Å): | 6.20 |
| Reference: | 10.1038/ncomms14722 |
| Antibody | |
| Antibody: | ZV67 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Zika Virus envelope protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: J
Mutation: NULL
| >5UHY_J|Chain B[auth H], D[auth J]|ZV67 Fab chain 2|Homo sapiens (9606) AQLQQSGTGLARPGASVKLSCKASGYTFTSYGISWVTQRAGQGLEWIGVIYPRSGNTYYNEKFRGKATLTADKSSSSAYMELRGLTAEDSAVYFCARENYGSVYWGQGTTLTVSSAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIEPRVPI |
Light Chain: I
Mutation: NULL
| >5UHY_I|Chain A[auth G], C[auth I]|ZV67 Fab chain 1|Homo sapiens (9606) DIVMTQSQKFMSTSVGDRVSITCKASQNVGTAVAWYQQKPGQSPKLLIYSASNRYTGVPDRFTGSGSGTDFTLTISNMQSEDLADYFCQQFSSYPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: NULL
| >5UHY_C|Chain E[auth A], F[auth C], G[auth E]|envelope protein|Zika virus (64320) IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition I: ALA32 LEU46 TYR49 SER50 ASN53 ARG54 TYR55 THR56 SER92 J: ALA2 GLY26 TYR27 THR28 THR30 SER31 TYR32 GLY33 ARG53 SER54 TYR58 ARG94 GLU95 ASN96 TYR97 GLY98 VAL101 TYR102 C: ILE65 SER66 ASP67 MET68 ALA69 LEU82 ASP83 LYS84 ASP87 GLN89 TYR90 LYS118 ALA120 CYS121 SER122 GLY232 THR233 THR254 VAL255 VAL256 VAL257 LEU258 GLY259 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
