Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 173 |
| PDBID: | 1UAC |
| Chains: | HL_Y |
| Organism: | Mus musculus, Meleagris gallopavo |
| Method: | XRD |
| Resolution (Å): | 1.70 |
| Reference: | 10.1074/jbc.M301149200 |
| Antibody | |
| Antibody: | HyHEL-10 Fv |
| Antibody mutation: | Yes |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Turkey Egg White Lysozyme |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: Y53S/S54F/Y58F
| >1UAC_H|Chain B[auth H]|Ig VH,anti-lysozyme|Mus musculus (10090) DVQLQESGPSLVKPSQTLSLTCSVTGDSITSDYWSWIRKFPGNRLEYMGYVSSFGSTFYNPSLKSRISITRDTSKNQYYLDLNSVTTEDTATYYCANWDGDYWGQGTLVTVSAA |
Light Chain: L
Mutation: NULL
| >1UAC_L|Chain A[auth L]|lysozyme binding Ig kappa chain V23-J2 region|Mus musculus (10090) DIVLTQSPATLSVTPGNSVSLSCRASQSIGNNLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGTDFTLSINSVETEDFGMYFCQQSNSWPYTFGGGTKLEIK |
Antigen
Chain: Y
Mutation: NULL
| >1UAC_Y|Chain C[auth Y]|Lysozyme C|Meleagris gallopavo (9103) KVYGRCELAAAMKRLGLDNYRGYSLGNWVCAAKFESNFNTHATNRNTDGSTDYGILQINSRWWCNDGRTPGSKNLCNIPCSALLSSDITASVNCAKKIASGGNGMNAWVAWRNRCKGTDVHAWIRGCRL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: GLY30 ASN31 ASN32 LYS49 TYR50 SER52 GLN53 SER67 SER91 ASN92 SER93 TRP94 TYR96 H: ASP27 THR30 SER31 ASP32 TYR33 TYR50 SER52 SER53 PHE54 SER56 PHE58 ASN97 TRP98 ASP99 Y: LYS13 ARG14 LEU15 GLY16 ASP18 ASN19 TYR20 ARG21 TRP62 TRP63 SER72 LYS73 ASN74 LEU75 ASN77 THR89 ASN93 LYS96 LYS97 SER100 GLY101 GLY102 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
