Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1725 |
| PDBID: | 5UEM |
| Chains: | HL_G |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1126/scitranslmed.aal2144 |
| Antibody | |
| Antibody: | 354NC37 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 clade AE strain 93TH057 gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5UEM_H|Chain B[auth H]|354NC37 Fab Heavy Chain|Homo sapiens (9606) QVRLVQSGGQVRKPGASVTISCEADGYEFPDYYIHWVRLARGRGPEWLGLIKVGHGGGAMYAPSLQGRISMSRDIHTTTAYMTLQRLTHDDTATYYCSRDNFGTRPVPGRGYYYGMDVWGQGTAIIVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPK |
Light Chain: L
Mutation: NULL
| >5UEM_L|Chain C[auth L]|354NC37 Fab Light Chain|Homo sapiens (9606) EIVLTQSPGILSLAPGERASLSCRASYGLDTSHLAWFQHKPGRPPRLLIYGTSSRPPGVPDRFRGSGSGTDFTLTITKLEPEDFAVYYCQNSGGGTPLIFGPGTKVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR |
Antigen
Chain: G
Mutation: NULL
| >5UEM_G|Chain A[auth G]|clade A/E 93TH057 HIV-1 gp120 core|Human immunodeficiency virus 1 (11676) VWKDADTTLFCASDAKAHETECHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVEQMQEDVISLWDQCLQPCVKLTGGSVIKQACPKISFDPIPIHYCTPAGYVILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKTIIVHLNKSVEINCTRPSNGGSGSGGDIRKAYCEINGTKWNKVLKQVTEKLKEHFNNKTIIFQPPSGGDLEITMHHFNCRGEFFYCNTTQLFNNTCIGNETMKGCNGTITLPCKIKQIINMWQGTGQAMYAPPIDGKINCVSNITGILLTRDGGANNTSNETFRPGGGNIKDNWRSELYKYKVVQIEGSHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: PRO30 GLY52 HIS53 GLY54 GLY55 GLY56 ALA57 MET58 PRO61 ARG71 ASP72 ILE73 HIS74 THR76 PHE97 GLY98 THR99 L: GLU1 ASP29 HIS32 GLY92 GLY93 GLY94 G: TRP96 LYS97 ASN98 ASN99 GLU102 LEU122 GLY124 GLY198 VAL200 ASN276 ASN279 ASN280 ALA281 LYS282 SER365 GLY366 GLY367 ASP368 ILE371 ASN425 TRP427 GLN428 GLY429 THR430 GLY431 GLN432 THR455 GLY458 GLY459 ASN461 GLY472 GLY473 ASN474 LYS476 ARG480 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
