Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1724 |
| PDBID: | 5UEK |
| Chains: | HL_A |
| Organism: | Saccharomyces cerevisiae S288C, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.70 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | 12E Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Histone chaperone ASF1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5UEK_H|Chain B[auth H]|Fab 12E Heavy Chain|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFNVSYYSIHWVRQAPGKGLEWVASIYPYSGSTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARGYGWALDYWGQGTLVTVFNQIKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: L
Mutation: NULL
| >5UEK_L|Chain C[auth L]|Fab 12E Light Chain|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQDGWSLITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >5UEK_A|Chain A|Histone chaperone ASF1|Saccharomyces cerevisiae (559292) GSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSILVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEEELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: TYR31 TYR32 SER33 PRO52 TYR53 SER54 SER56 THR57 SER58 GLY95 TYR96 GLY97 TRP98 L: GLN27 SER28 VAL29 SER30 SER31 TYR49 SER50 SER65 ARG66 SER67 GLY68 ASP91 GLY92 TRP93 SER94 A: SER5 LEU6 LEU7 GLY8 ILE9 VAL45 SER47 SER48 ARG49 SER50 LEU51 ASP52 VAL92 THR93 VAL94 LEU96 ARG108 GLY110 TYR111 TYR112 ASN114 ASN138 LEU140 GLU142 LYS143 PRO144 ARG145 VAL146 THR147 ARG148 PHE149 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
