Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1707 |
| PDBID: | 5TZT |
| Chains: | AB_D |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.89 |
| Reference: | 10.1038/bcj.2017.7 |
| Antibody | |
| Antibody: | C47B161 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Leukocyte surface antigen CD47 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >5TZT_A|Chain B[auth A], D[auth H]|Heavy Chain of Fab C47B161|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKASGYTFTDYNMHWVRQAPGQRLEWMGDIYPYNGGTGYNQKFKGRVTITRDTSASTAYMELSSLRSEDTAVYYCARGGWHAMDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHH |
Light Chain: B
Mutation: NULL
| >5TZT_B|Chain A[auth B], C[auth L]|Light Chain of Fab C47B161|Homo sapiens (9606) DIVMTQSPLSLPVTPGEPASISCRSRQSIVHTNRYTYLAWYLQKPGQSPQLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCFQGSHVPYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: D
Mutation: NULL
| >5TZT_D|Chain E[auth C], F[auth D]|Leukocyte surface antigen CD47|Homo sapiens (9606) QLLFNKTKSVEFTFGNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNEHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: TYR35 TYR37 LEU51 TYR54 LYS55 ASN58 ARG59 PHE60 SER61 A: THR30 ASP31 TYR32 TYR52 TYR54 GLY99 GLY100 TRP101 HIS102 ALA103 ASP105 D: LEU2 LEU3 ASN27 GLU29 GLU35 TYR37 LYS39 ASP51 THR99 GLU100 LEU101 THR102 ARG103 GLU104 GLY105 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
