Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1705 |
| PDBID: | 5TZ2 |
| Chains: | HL_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1038/bcj.2017.7 |
| Antibody | |
| Antibody: | C47B222 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Leukocyte surface antigen CD47 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5TZ2_H|Chain A[auth H]|C47B222 Fab Heavy Chain|Homo sapiens (9606) EVQLVQSGAEVKKPGESLKISCKGSGYSFTDYWIGWVRQMPGKGLEWMGIIYPGDSDTRYSPSFQGQVTISADKSISTAYLQWSSLKASDTAVYYCARVGRFASHQLDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHH |
Light Chain: L
Mutation: NULL
| >5TZ2_L|Chain B[auth L]|C47B222 Fab Light Chain|Homo sapiens (9606) EIVLTQSPATLSLSPGERATLSCRASQSVNNRLAWYQQKPGQAPRLLIHWASTRAIGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQQGASWPFTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >5TZ2_C|Chain C|Leukocyte surface antigen CD47|Homo sapiens (9606) QLLFNKTKSVEFTFGNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNEHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ASP31 TRP33 ILE50 TYR52 ASP55 ASP57 ARG59 ARG101 PHE102 ALA103 HIS105 L: ASN30 ASN31 ARG32 TRP50 SER67 ALA92 SER93 TRP94 PHE96 C: THR34 GLU35 VAL36 TYR37 LYS39 ASP46 THR49 ASP51 ALA53 LEU54 ASN55 LYS56 THR58 VAL59 PRO60 GLU97 THR99 LEU101 THR102 GLU104 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
