Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 170 |
| PDBID: | 1TZH |
| Chains: | BA_W |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1073/pnas.0401786101 |
| Antibody | |
| Antibody: | YADS1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Vascular endothelial growth factor A |
| Antigen mutation: | No |
| Durg Target: | P15692 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >1TZH_B|Chain D[auth B], F[auth H]|Fab YADS1 Heavy Chain|Mus musculus (10090) EVQLVESGGGLVQPGGSLRLSCAASGFDIYDDDIHWVRQAPGKGLEWVAYIAPSYGYTDYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRSSDASYSYSAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH |
Light Chain: A
Mutation: NULL
| >1TZH_A|Chain C[auth A], E[auth L]|Fab YADS1 Light Chain|Mus musculus (10090) DIQMTQSPSSLSASVGDRVTITCRASQASYSSVAWYQQKPGKAPKLLIYAASYLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQSSASPATFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: W
Mutation: NULL
| >1TZH_W|Chain A[auth V], B[auth W]|Vascular endothelial growth factor A|Homo sapiens (9606) GQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER29 TYR30 SER31 SER32 TYR49 ALA50 ALA51 TYR53 SER67 GLY68 SER92 ALA93 SER94 B: ASP33 TYR50 SER53 TYR54 TYR56 W: TYR45 ILE46 LYS48 GLN79 MET81 ARG82 ILE83 LYS84 PRO85 HIS86 GLN87 GLY88 GLN89 HIS90 ILE91 GLY92 GLU93 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
