Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 17 |
| PDBID: | 1C08 |
| Chains: | BA_C |
| Organism: | Mus musculus, Gallus gallus |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1074/jbc.274.39.27623 |
| Antibody | |
| Antibody: | HYHEL-10 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hen Egg White Lysozyme |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >1C08_B|Chain B|ANTI-HEN EGG WHITE LYSOZYME ANTIBODY (HYHEL-10)|Mus musculus (10090) DVQLQESGPSLVKPSQTLSLTCSVTGDSITSDYWSWIRKFPGNRLEYMGYVSYSGSTYYNPSLKSRISITRDTSKNQYYLDLNSVTTEDTATYYCANWDGDYWGQGTLVTVSAA |
Light Chain: A
Mutation: NULL
| >1C08_A|Chain A|ANTI-HEN EGG WHITE LYSOZYME ANTIBODY (HYHEL-10)|Mus musculus (10090) DIVLTQSPATLSVTPGNSVSLSCRASQSIGNNLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGTDFTLSINSVETEDFGMYFCQQSNSWPYTFGGGTKLEIK |
Antigen
Chain: C
Mutation: NULL
| >1C08_C|Chain C|LYSOZYME|Gallus gallus (9031) KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: GLN27 SER28 GLY30 ASN31 ASN32 LYS49 TYR50 GLN53 SER91 ASN92 SER93 TRP94 TYR96 B: THR30 SER31 ASP32 TYR33 TYR50 SER52 TYR53 SER54 SER56 TYR58 TRP98 ASP99 ASP101 C: LYS13 ARG14 HIS15 GLY16 ASP18 ASN19 TYR20 ARG21 TRP62 TRP63 ARG73 ASN74 LEU75 ASN77 THR89 ASN93 LYS96 LYS97 SER100 ASP101 GLY102 ASN103 ALA107 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
