Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1696 |
| PDBID: | 5TOK |
| Chains: | E_A |
| Organism: | human respiratory syncytial virus, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.80 |
| Reference: | 10.1038/ncomms14158 |
| Antibody | |
| Antibody: | F-VHH-L66 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | respiratory syncytial virus Fusion glycoprotein F0 |
| Antigen mutation: | No |
| Durg Target: | P03420; P11209; |
Sequence information
Antibody
Chain: E
Mutation: NULL
| >5TOK_E|Chain D, E, F|Single-domain antibody F-VHH-L66|Lama glama (9844) QVQLQESGGGLVQPGGSLRLSCAASGFTLDYYYIGWFRQAPGKEREGVSCISSSHGSTYYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCATVAVAHFRGCGVDGMDYWGKGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >5TOK_A|Chain A, B, C|Fusion glycoprotein F0, Fibritin chimera|Human respiratory syncytial virus (11250) MELLILKANAITTILTAVTFCFASGQNITEEFYQSTCSAVSKGYLSALRTGWYTSVITIELSNIKENKCNGTDAKVKLIKQELDKYKNAVTELQLLMQSTPATNNRARRELPRFMNYTLNNAKKTNVTLSKKRKRRFLGFLLGVGSAIASGVAVCKVLHLEGEVNKIKSALLSTNKAVVSLSNGVSVLTFKVLDLKNYIDKQLLPILNKQSCSISNIETVIEFQQKNNRLLEITREFSVNAGVTTPVSTYMLTNSELLSLINDMPITNDQKKLMSNNVQIVRQQSYSIMCIIKEEVLAYVVQLPLYGVIDTPCWKLHTSPLCTTNTKEGSNICLTRTDRGWYCDNAGSVSFFPQAETCKVQSNRVFCDTMNSLTLPSEVNLCNVDIFNPKYDCKIMTSKTDVSSSVITSLGAIVSCYGKTKCTASNKNRGIIKTFSNGCDYVSNKGVDTVSVGNTLYYVNKQEGKSLYVKGEPIINFYDPLVFPSDEFDASISQVNEKINQSLAFIRKSDELLSAIGGYIPEAPRDGQAYVRKDGEWVLLSTFLGGLVPR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: TYR31 TYR33 GLU44 ARG45 GLU46 CYS50 HIS54 SER56 THR57 TYR58 TYR59 LYS64 ALA96 VAL97 ALA98 HIS99 A: THR50 GLY51 TRP52 TYR53 SER180 SER182 ASN183 GLY184 VAL185 SER186 MET264 PRO265 ILE266 THR267 ASN268 ASP269 GLN270 LYS271 LEU305 GLY307 VAL308 ILE309 ASP310 THR311 ASN345 ALA346 GLY347 THR369 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
