Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1690 |
| PDBID: | 5TLK |
| Chains: | FE_Y |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1016/j.molimm.2017.01.005 |
| Antibody | |
| Antibody: | M2177 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | CD27 antigen |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >5TLK_F|Chain B, F|M2177 HEAVY CHAIN|Mus musculus (10090) QVQLQQSGPELVKPGASVKISCKASGYAFSSSWMNWVKQRPGKGLEWIGRIYPGDGDTNYNGKFKGKATLTADKSSSTAYMQLSSLTSEDSAVYFCARSDYYGDYGFAYWGQGTLVTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHH |
Light Chain: E
Mutation: NULL
| >5TLK_E|Chain A, E|M2177 LIGHT CHAIN|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCKASQSVDYAGDSYMNWYQQKPGQPPKLLIYAASNLESGIPARFSGSGSGTDFTLNIHPVEEEDAATYYCQQSNEDPYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: Y
Mutation: NULL
| >5TLK_Y|Chain I[auth X], J[auth Y]|CD27 antigen|Homo sapiens (9606) TPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDGGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: TYR31 ALA32 ASP34 TYR36 TYR53 ASN57 SER95 ASN96 F: SER31 SER32 TRP33 TYR52 ASP55 ASP57 TYR101 TYR102 ASP104 TYR105 Y: LYS5 SER6 CYS7 PRO8 GLU9 HIS11 TRP13 GLN15 GLY16 LYS17 LEU18 CYS19 GLN21 HIS36 ARG37 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
