Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1687 |
| PDBID: | 5TLJ |
| Chains: | DC_X |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.50 |
| Reference: | 10.1016/j.molimm.2017.01.005 |
| Antibody | |
| Antibody: | M2191 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | CD27 antigen |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >5TLJ_D|Chain D, H|M2191 HEAVY CHAIN|Mus musculus (10090) EVKLVESGGGLVKPGGSLKLSCAASGFTFSSYTMSWVRQTPEKRLEWVAYISSGGGNTYYPDSVKGRFTISRDNARNTLYLQMSSLRSEDTAMYYCSRHRGNPFDYWGQGTTLTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHH |
Light Chain: C
Mutation: NULL
| >5TLJ_C|Chain C, G|M2191 LIGHT CHAIN|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCRASKSVSTSGYSFMHWYQQKPGQPPKLLIYLASNLESGVPARFSGSGSGTDFTLNIHPVEEEDAATYYCQHSRELPWTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: X
Mutation: NULL
| >5TLJ_X|Chain I[auth X]|CD27 antigen|Homo sapiens (9606) TPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDGGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: THR31 SER32 TYR34 PHE36 TYR53 LEU54 SER60 SER95 ARG96 GLU97 LEU98 TRP100 D: SER31 TYR32 THR33 TYR50 SER52 SER53 GLY56 ASN57 THR58 TYR59 HIS99 ARG100 GLY101 ASN102 X: GLU24 THR27 PHE28 VAL30 GLN41 CYS42 ASP43 PRO44 CYS45 ILE46 PRO47 GLY48 VAL49 PRO59 HIS60 SER63 CYS64 HIS66 THR77 ILE78 THR79 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
