Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1684 |
| PDBID: | 5TIH |
| Chains: | HL_A |
| Organism: | Plasmodium falciparum 3D7, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.44 |
| Reference: | 10.7554/eLife.21347 |
| Antibody | |
| Antibody: | CyRPA Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Cysteine-rich protective antigen |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5TIH_H|Chain B[auth H]|CyRPA antibody Fab Heavy Chain|Mus musculus (10090) QVQLQQSGPQLVRPGASVKISCKTSGYSFTSFWMHWVKQWPGQGLEWIGMIDPSENKIRLNQNFKDRATLTVDTSSATAYIQFSRPTSEDSGVYYCAMVRRGYWGQGTTLTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRD |
Light Chain: L
Mutation: NULL
| >5TIH_L|Chain C[auth L]|CyRPA antibody Fab Light Chain|Mus musculus (10090) DIQMTQSPSSLSASLGERVSLTCRASQEISGYLSWLQRKPDGTIKRLIYTASTVDSGVPNRFSGSRSGSDYSLTISSLESEDFADYYCLQYDTYPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN |
Antigen
Chain: A
Mutation: NULL
| >5TIH_A|Chain A|Cysteine-rich protective antigen|Plasmodium falciparum (36329) GTSRHVFIRTELSFIKNNVPCIRDMFFIYKRELYNICLDDLKGEEDETHIYVQKKVKDSWITLNDLFKETDLTGRPHIFAYVDVEEIIILLCEDEEFSNRKKDMTCHRFYSNDGKEYNNAEITISDYILKDKLLSSYVSLPLKIENREYFLICGVSPYKFKDDNKKDDILCMASHDKGETWGTKIVIKYDNYKLGVQYFFLRPYISKNDLSFHFYVGDNINNVKNVNFIECTHEKDLEFVCSNRDFLKDNKVLQDVSTLNDEYIVSYGNDNNFAECYIFFNNENSILIKPEKYGNTAAGCYGGTFVKIDENRALFIYSSSQGIYNIHTIYYANYE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR27 SER28 THR30 SER31 PHE32 TRP33 HIS35 ASP52 SER54 GLU55 LYS57 ARG59 MET98 ARG100 ARG101 TYR103 L: TYR32 TYR49 THR50 THR53 TYR91 ASP92 TYR94 A: LYS40 GLY41 GLU42 ASP44 GLU45 THR46 ASN62 ASP63 PHE65 LYS66 GLU67 THR68 ASP69 LEU70 THR71 GLY72 GLU91 ASP92 GLU93 LYS99 THR103 HIS105 TYR115 ASN116 ASN117 ALA118 GLU119 THR121 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
