Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1681 |
| PDBID: | 5THR |
| Chains: | PQ_C |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 8.90 |
| Reference: | 10.1073/pnas.1615939113 |
| Antibody | |
| Antibody: | 8ANC195 G52K5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | BG505 SOSIP gp41 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: P
Mutation: NULL
| >5THR_P|Chain P, R, T|8ANC195 G52K5 VH domain|Homo sapiens (9606) QIHLVQSGTEVKKPGSSVTVSCKAYGVNTFGLYAVNWVRQAPGQSLEYIGQIWRWKSSASHHFRGRVLISAVDLTGSSPPISSLEIKNLTSDDTAVYFCTTTSTYDKWSGLHHDGVMAFSSWGQGTLISVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHHHHHH |
Light Chain: Q
Mutation: NULL
| >5THR_Q|Chain Q, S, U|8ANC195 G52K5 VL domain|Homo sapiens (9606) DIQMTQSPSTLSASIGDTVRISCRASQSITGNWVAWYQQRPGKAPRLLIYRGAALLGGVPSRFSGSAAGTDFTLTIGNLQAEDFGTFYCQQYDTYPGTFGQGTKVEVKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >5THR_C|Chain A, B, C|BG505 SOSIP gp41|Human immunodeficiency virus 1 (11676) AVGIGAVFLGFLGAAGSTMGAASMTLTVQARNLLSGIVQQQSNLLRAPEAQQHLLKLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICCTNVPWNSSWSNRNLSEIWDNMTWLQWDKEISNYTQIIYGLLEESQNQQEKNEQDLLALD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| P: TRP53 ARG54 ASP106 LYS107 TRP108 SER109 GLY110 LEU111 HIS112 HIS113 ASP114 GLY115 Q: GLN27 SER28 ILE29 THR30 GLY31 TRP33 ARG51 ALA67 GLY69 THR70 PHE72 ASP93 C: SER528 THR529 TRP610 SER612 SER613 TRP614 SER615 ASN616 GLU621 TRP628 LEU629 GLN630 TRP631 ASP632 LYS633 GLU634 ILE635 TYR638 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
