Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 168 |
| PDBID: | 1TQC |
| Chains: | BC_A |
| Organism: | Ovis aries, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1073/pnas.0400014101 |
| Antibody | |
| Antibody: | VRQ14 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | prion protein(114-234) ARR variant |
| Antigen mutation: | No |
| Durg Target: | P04156 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >1TQC_B|Chain B|VRQ14 Fab Heavy chain|Mus musculus (10090) QIQLVQSGPELKKPGETVKISCKASGYTFTNYGMNLVKQAPGKGFEWMGWINTFTGEPTYADDFKGRFVFSLDTSASTAYLQINNLKNEDTATYFFTRGTDYWGQGTTLTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEP |
Light Chain: C
Mutation: NULL
| >1TQC_C|Chain C|VRQ14 Fab light chain|Mus musculus (10090) DVVMSQTPLTLSVTIGQPASISCKSSQSLLDSDGKTYLNWLLQRPGQSPKRLIYLVSRLDSGVPDRFTGSGSGTDFTLKISRVEAEDLGIYFCWQGSHFPQTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >1TQC_A|Chain A|prion protein|Ovis aries (9940) GLGGYMLGSAMSRPLIHFGNDYEDRYYRENMYRYPNQVYYRPVDRYSNQNNFVHDCVNITVKQHTVTTTTKGENFTETDIKIMERVVEQMCITQYQRESQAY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: THR28 THR30 ASN31 TYR32 GLY33 ASN35 TRP50 THR52 PHE53 THR54 ARG94 GLY95 THR96 ASP101 C: ASP29 LYS30 TYR32 ASN34 ARG46 TYR49 LEU50 ARG53 LEU54 ASP55 SER56 GLY91 SER92 HIS93 GLN96 A: GLY127 LEU128 TYR152 ASN156 TYR158 ARG159 ILE185 VAL187 LYS188 GLN189 HIS190 THR191 VAL192 THR193 THR194 THR195 THR196 LYS197 GLY198 GLU199 ASN200 PHE201 THR202 ASP205 ILE206 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
