Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1671 |
| PDBID: | 5TE4 |
| Chains: | HL_G |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.75 |
| Reference: | 10.1016/j.immuni.2016.10.027 |
| Antibody | |
| Antibody: | N6 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 clade G strain X2088 gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5TE4_H|Chain B[auth H]|Heavy chain of antibody N6|Homo sapiens (9606) RAHLVQSGTAMKKPGASVRVSCQTSGYTFTAHILFWFRQAPGRGLEWVGWIKPQYGAVNFGGGFRDRVTLTRDVYREIAYMDIRGLKPDDTAVYYCARDRSYGDSSWALDAWGQGTTVVVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >5TE4_L|Chain C[auth L]|Light chain of antibody N6|Homo sapiens (9606) YIHVTQSPSSLSVSIGDRVTINCQTSQGVGSDLHWYQHKPGRAPKLLIHHTSSVEDGVPSRFSGSGFHTSFNLTISDLQADDIATYYCQVLQFFGRGSRLHIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: G
Mutation: NULL
| >5TE4_G|Chain A[auth G]|HIV-1 clade G strain X2088 gp120|Human immunodeficiency virus 1 (11676) AWEDADTTLFCASDAKAYSTEKHNVWATHACVPTDPDPQEIPLENVTENFNMWKNNMVEQMHEDIISLWDESLKPCVMLTGGSTIKQACPKVTFEPIPIHYCAPAGFAILKCRDEDFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAKGDIVIRSENLTNNAKVIIVQLNEPVQIVCIRPNNGGSGSGGDIRQAHCNVTRGKWVNITKNVKEQLWKIFNKTTNITFNNTIFNSPAGGDLEITTHSFNCGGEFFYCNTSDLFNETNLSANHTDTNENITLQCRIKQIVRMWQRVGQAMYAPPIAGNITCISNITGLLLTRDGVNDTHDKENETFRPTGGDMRDNWRSELYKYKVIKLK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR30 ILE33 PHE35 GLU46 TRP47 TRP50 GLN53 TYR54 GLY55 ALA56 VAL57 ASN58 PHE59 GLY60 GLY61 GLY62 ARG64 ARG71 VAL73 TYR74 GLY99 L: TYR1 ILE2 HIS3 GLN27 ASP32 LEU91 GLN96 PHE97 G: LYS97 GLY124 GLY198 GLU275 ASN276 THR278 ASN279 ASN280 ALA281 LYS282 VAL283 ALA365 GLY366 GLY367 ASP368 GLU370 ILE371 ARG425 MET426 TRP427 VAL430 THR455 ARG456 ASP457 GLY458 VAL459 ASN460 ASP461 THR462 ARG469 THR471 GLY472 GLY473 ASP474 MET475 ARG476 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
