Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1665 |
| PDBID: | 5T5N |
| Chains: | ML_C |
| Organism: | Gallus gallus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.1073/pnas.1614688113 |
| Antibody | |
| Antibody: | 10D10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | bestrophin-1 (BEST1) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >5T5N_M|Chain G, I, K, M, O|Fab antibody fragment, heavy chain (10D10)|Mus musculus (10090) QVQLQQSGPELVRPGASVKMSCKASGYTFTNYWMHWVKQRPGQALEWIGMIDPSKSETTLNQKFRGKATLNVDKSSNTAYMQLSSLTSEDSAVYYCAREVYYFDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSSWPSETVTCNVAHPASSTKVDKKIVPRD |
Light Chain: L
Mutation: NULL
| >5T5N_L|Chain F, H, J, L, N|Fab antibody fragment, light chain (10D10)|Mus musculus (10090) DIQMTQSPASLSASVGETVTITCRASENIYSYLTWYQQKQGKSPQLLVYNAKTLTEGVPSRFSGSGSGTQFSLKINSLQPEDFGGYFCQHHYGTPPTFGGGTKLEVKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN |
Antigen
Chain: C
Mutation: NULL
| >5T5N_C|Chain A, B, C, D, E|bestrophin-1 (BEST1)|Gallus gallus (9031) TVTYTNRVADARLGTFSQLLLQWKGSIYKLLYSEFLIFISLYFAISLVYRLILSESQRLMFEKLALYCNSYAELAPVSAVLGAYVSLVVSRWWAQYESIPWPDRIMNLVSCNVDGEDEYGRLLRRTLMRYSNLCSVLILRSVSTAVYKRFPSMEHVVRAGLMTPEEHKKFESLNSPHNKFWIPCVWFSNLAVKARNEGRIRDSVLLQGILNELNTLRSQCGRLYGYDWISIPLVYTQVVTVAVYSFFLACLIGRQFLDPEKAYPGHELDLFVPVFTFLQFFFYAGWLKVAEQLINPFGEDDDDFETNWLIDRNLQVSLMAVDEMHQDLPILEKDLYWNEPDPQPPYTAATAEYKRPSFLGSTFDISMQKEEMEFQPLEQIKENEEANHSTPLLGHLGRLLGVQSEGEEF |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR30 SER31 TYR32 TYR49 ASN50 THR53 HIS91 TYR92 GLY93 THR94 PRO96 M: TRP33 HIS35 MET50 LYS55 GLU99 VAL100 TYR101 C: ASN7 LEU336 TYR337 GLN344 PRO345 PRO346 TYR347 THR348 ALA349 ALA350 ALA352 LYS355 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
