Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1653 |
| PDBID: | 5T33 |
| Chains: | HL_G |
| Organism: | Homo sapiens, Human immunodeficiency virus |
| Method: | XRD |
| Resolution (Å): | 3.21 |
| Reference: | 10.1128/JVI.01357-16 |
| Antibody | |
| Antibody: | CAP257-RH1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 strain RHPA gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5T33_H|Chain A[auth H]|CAP257-RH1 heavy chain|Homo sapiens (9606) QVQLVESGGGLFQPGRSLRLSCVASGFSFNEKAMHWVRQAPGKGLEWVAIIWYNSIHKNYADSVKGRFTISRDNSKNTLYLQMNSLRADDTAVYYCANAGGGKYDYLDVWGRGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >5T33_L|Chain B[auth L]|CAP257-RH1 light chain|Homo sapiens (9606) SYELTQPPSVSVSPGQTARITCSGDTLSTKWSYWCQQKSGQAPVLVIYEGNKRFSGTPAKFSGFHSGTMATLTISGAQVDDEADYYCFSTDSSGQTWVFGGGTKLTVLRQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: G
Mutation: NULL
| >5T33_G|Chain C[auth G]|RHPA gp120 core|Human immunodeficiency virus (12721) VWKEANTTLFCASDAKAYDTEAHNVWATHACVPTDPNPQEVVLENVTENFNMWKNHMVEQMHEDIISLWDQSLKPCVKLTGGVITQACPKISFEPIPIHYCAPAGFAILKCNDKKFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSENFTNNVKNIIVQLNESVQINCTRHNNGGSGSGGDIRQAHCNISREKWQNTLKQIVKKLREQFKNKTIAFAPSSGGDPEIVMHSFNCNGEFFYCNTTKLFTSTWNSTWNSTWNNTEGSNSTVITLPCRIRQIINMWQEVGKAMYAPPIQGQIKCSSNITGLLLTRDGGVDTTKETFRPGGGNMKDNWRSELYKYKVVRIE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLU31 LYS32 TYR52 ILE55 HIS56 GLY97 GLY98 LYS99 L: SER29 THR30 LYS31 TRP32 TYR34 GLU50 LYS53 HIS66 SER90 THR91 TRP96 G: ASN279 ASN280 VAL281 LYS282 LYS354 LYS357 SER365 GLY366 GLY367 ASP368 THR455 ARG456 ASP457 GLY458 GLY459 VAL460 ASP461 THR462 THR463 LYS465 ARG469 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
