Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1638 |
| PDBID: | 5OTJ |
| Chains: | BA_D |
| Organism: | Homo sapiens, Phleum pratense |
| Method: | XRD |
| Resolution (Å): | 2.35 |
| Reference: | 10.1073/pnas.1806840115 |
| Antibody | |
| Antibody: | 102.1F10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Polcalcin Phl p 7 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >5OTJ_B|Chain B, E[auth H]|102.1F10 Fab heavy chain|Homo sapiens (9606) QVQLVQSGAEVRNPGASVKVSCKASGYTFTSYAIHWVRQAPGHRLEWVGRINTDNGNTKYSQKFHGRVALSRDTSASTTYMDLSSLNSEDTAVYYCARAFYYSSGVMFDSWGQGALVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHH |
Light Chain: A
Mutation: NULL
| >5OTJ_A|Chain A, C[auth L]|102.1F10 Fab light chain|Homo sapiens (9606) QSALTQPPSVSGAPGQRVSISCTGGSSNFGAGYDVHWYQQLPATAPKLLIYGNNNRPSGVPDRFSGSKSGTSASLAITGLQAEDEGDYFCQSFDTSLSGWIFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: D
Mutation: NULL
| >5OTJ_D|Chain D[auth C], F[auth D]|Polcalcin Phl p 7|Phleum pratense (15957) MADDMERIFKRFDTNGDGKISLSELTDALRTLGSTSADEVQRMMAEIDTDGDGFIDFNEFISFCNANPGLMKDVAKVFKGELNSKLEGKPIPNPLLGLDSTRTGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ALA31 GLY32 TYR33 PHE93 SER98 TRP100 B: THR28 THR30 SER31 TYR32 ALA33 HIS35 ARG50 ASN52 ASP54 ASN55 LYS59 PHE100 TYR101 TYR102 SER103 SER104 GLY105 VAL106 D: LYS19 SER21 LEU22 SER23 ASP38 GLN41 ARG42 MET44 ALA45 GLU46 ASP48 THR49 ASP50 GLY51 ASP52 GLY53 PHE54 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
