Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1615 |
| PDBID: | 5NQW |
| Chains: | D_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.40 |
| Reference: | 10.1038/s41467-017-02312-7 |
| Antibody | |
| Antibody: | 026 VH-sdAb |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Immunoglobulin heavy constant epsilon |
| Antigen mutation: | No |
| Durg Target: | P01854 |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >5NQW_D|Chain C, D|026|Lama glama (9844) MEVQLLESGGGLVQPGGSLRLSCAASGFTFGNYDMAWVRQAPGKRPEWVSSIDTGGDITHYADSVKGRFTISRDNAKNTLYLQMNSLRPEDTAVYWCATDEEYALGPNEFDYYGQGTLVTVSSAAALEHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >5NQW_A|Chain A, B|Immunoglobulin heavy constant epsilon|Homo sapiens (9606) RGVSAYLSRPSPFDLFIRKSPTITCLVVDLAPSKGTVNLTWSRASGKPVNHSTRKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGPRAAPEVYAFATPEWPGSRDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKGSGFFVFSRLEVTRAEWEQKDEFICRAVHEAASPSQTVQRAVSVNP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: GLU2 VAL38 GLN40 ARG45 PRO46 TRP96 ASP100 GLU102 TYR103 PRO107 ASN108 GLU109 PHE110 ASP111 TYR113 GLY114 GLN115 A: SER432 SER437 GLY438 PRO439 ARG440 ALA441 ALA442 GLU444 ASN468 MET470 LYS497 LYS499 GLY500 SER501 GLY502 PHE503 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
