Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1614 |
| PDBID: | 5NMV |
| Chains: | HL_K |
| Organism: | Mus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.65 |
| Reference: | 10.1111/jth.14000 |
| Antibody | |
| Antibody: | 2F22 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tissue factor pathway inhibitor |
| Antigen mutation: | No |
| Durg Target: | P10646 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5NMV_H|Chain B[auth H]|Fab heavy chain|Mus (10088) QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLAVIWRGGSIDYNAAFMSRLSITKDNSKSQVFFKMNSLQADDTAIYYCAKNSHGNYVGYAMDYWGQGTSVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESK |
Light Chain: L
Mutation: NULL
| >5NMV_L|Chain A[auth L]|Fab light chain|Mus (10088) GIVMTQSQKFMSTTVGDRVSITCKASQSVGPAVAWYQQKPGQSPKLLIYSASNRYTGVPDRFTGSGSGADFTLTISNLHSEDLADYFCQQYTSYPTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: K
Mutation: NULL
| >5NMV_K|Chain C[auth K]|Tissue factor pathway inhibitor|Homo sapiens (9606) GSSGSSGDSEEDEEHTIITDTELPPLKLMHSFCAFKADDGPCKAIMKRFFFNIFTRQCEEFIYGGCEGNQNRFESLEECKKMCTRD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: GLY1 PRO31 ALA32 TYR49 SER50 ASN53 TYR55 THR56 TYR91 THR92 SER93 TYR94 H: GLN1 VAL2 GLY26 PHE27 TYR32 TRP52 ARG53 GLY54 SER56 ILE57 ASP58 TYR59 ALA61 MET64 LYS97 SER99 HIS100 ASN102 TYR103 VAL104 GLY105 TYR106 ASP109 TYR110 K: GLU15 LEU16 PRO17 LEU19 LYS20 LEU21 MET22 SER24 PHE25 PRO34 CYS35 ALA37 MET39 LYS40 ARG41 TYR56 GLY58 CYS59 GLU60 GLY61 ASN62 GLN63 ARG65 PHE66 GLU67 SER68 GLU71 LYS74 MET75 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
