Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1610 |
| PDBID: | 5NJD |
| Chains: | UV_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.90 |
| Reference: | 10.1016/j.immuni.2017.12.008 |
| Antibody | |
| Antibody: | Briakinumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-12 subunit beta |
| Antigen mutation: | No |
| Durg Target: | P29460 |
Sequence information
Antibody
Heavy Chain: U
Mutation: NULL
| >5NJD_U|Chain M, O, Q, S, U, W|Briakinumab FAb Light chain|Homo sapiens (9606) MGILPSPGMPALLSLVSLLSVLLMGCVAQSVLTQPPSVSGAPGQRVTISCSGSRSNIGSNTVKWYQQLPGTAPKLLIYYNDQRPSGVPDRFSGSKSGTSASLAITGLQAEDEADYYCQSYDRYTHPALLFGTGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Light Chain: V
Mutation: NULL
| >5NJD_V|Chain N, P, R, T, V, X|Briakinumab FAb Heavy chain|Homo sapiens (9606) MGILPSPGMPALLSLVSLLSVLLMGCVAQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVAFIRYDGSNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCKTHGSHDNWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKGTSGLVPRGSGGSGGSGLNDIFEAQKIEWHEGRTKHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >5NJD_A|Chain A, C, E, G, I, K|Interleukin-12 subunit beta|Homo sapiens (9606) MCHQQLVISWFSLVFLASPLVAIWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| U: ASN32 THR33 LYS35 TYR50 TYR51 GLN90 TYR92 ARG94 TYR95 HIS97 V: GLN1 VAL2 GLY26 PHE27 THR28 SER31 TYR32 GLY33 ARG52 TYR53 SER56 ASN57 TYR59 THR98 HIS99 GLY100 SER101 HIS102 ASP103 ASN104 A: ASP36 TRP37 TYR38 PRO39 ASP40 ALA41 LYS80 GLU81 LYS106 LYS107 GLU108 ASP109 GLY110 ILE111 TRP112 SER113 THR114 ASP115 LEU117 LYS118 LYS121 LYS124 ASN125 LYS126 HIS216 LYS219 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
