Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1591 |
| PDBID: | 5N0A |
| Chains: | HL_A |
| Organism: | dengue virus type 2, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.90 |
| Reference: | 10.1038/ncomms15411 |
| Antibody | |
| Antibody: | EDE2 A11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | dengue virus type 2 envelope glycoprotein E |
| Antigen mutation: | Yes |
| Durg Target: | P27914; P12823; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5N0A_H|Chain C[auth H], D[auth I]|BROADLY NEUTRALIZING HUMAN ANTIBODY EDE2 A11 HEAVY CHAIN|Homo sapiens (9606) EVQLVESGGGLVRPGGSLRLSCAASGFSYSNHWMHWVRQAPGKGLVWVSRINSDGSTRNYADFVKGRFTISRDNAENTLYLEMNSLTADDTAVYYCVRDGVRFYYDSTGYYPDSFFKYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPLEDDDDKAGWSHPQFEKGGGSGGGSGGGSWSHPQFEK |
Light Chain: L
Mutation: NULL
| >5N0A_L|Chain E[auth L], F[auth M]|BROADLY NEUTRALIZING HUMAN ANTIBODY EDE2 A11|Homo sapiens (9606) RSQSVLTQPVSVSGSPGQSITISCTGTSSNADTYNLVSWYQQRPGKAPKLMIYEGTKRPSGVSNRFSASKSATAASLTISGLQPEDEADYYCCSYATSRTLVFGGGTKLTVVGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: A259C
| >5N0A_A|Chain A, B|Envelope Glycoprotein E|Dengue virus 2 (11060) MRCIGISNRDFVEGVSGGSWVDIVLEHGSCVTTMAKNKPTLDFELIKTEAKQPATLRKYCIEAKLTNTTTESRCPTQGEPSLNEEQDKRFICKHSMVDRGWGNGCGLFGKGGIVTCAKFTCKKNMEGKIVQPENLEYTIVITPHSGEEHAVGNDTGKHGKEIKITPQSSTTEAELTGYGTVTMECSPRTGLDFNEMVLLQMEDKAWLVHRQWFLDLPLPWLPGADTQGSNWIQKETLVTFKNPHAKKQDVVVLGSQEGCMHTALTGATEIQMSSGNLLFTGHLKCRLRMDKLQLKGMSYSMCTGKFKIVKEIAETQHGTIVIRVQYEGDGSPCKIPFEITDLEKRHVLGRLITVNPIVTEKDSPVNIEAEPPFGDSYIIVGVEPGQLKLNWFKKGLVPRGSSAWSHPQFEKGGSGGGSGGSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: ASP53 SER55 THR56 ARG57 ARG98 L: SER94 A: THR68 THR69 THR70 GLU71 SER72 ARG73 CYS74 LEU82 GLU84 VAL97 ASP98 ARG99 TRP101 GLY102 ASN103 GLY104 ILE113 LYS246 LYS247 GLN248 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
