Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1580 |
| PDBID: | 5MO9 |
| Chains: | HL_X |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.59 |
| Reference: | 10.1124/jpet.117.240184 |
| Antibody | |
| Antibody: | AB20 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | BDNF/NT-3 growth factors receptor |
| Antigen mutation: | No |
| Durg Target: | Q16620 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5MO9_H|Chain A[auth H]|AB20 Fab heavy chain|Mus musculus (10090) QVQLQQSGAELVRPGASVTLSCKASGYTFTDYEMHWVKQTPVHGLEWIGTIDPETAGTAYNQKFKGKAILTAGKSSSTAYMELRSLTSEDSAVYYCTGVTTWFAYWGQGTLVTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH |
Light Chain: L
Mutation: NULL
| >5MO9_L|Chain B[auth L]|AB20 Fab light chain|Mus musculus (10090) DVVMTQTPLSLPVSLGDQASISCRSSQSLVHSNGNTYLHWYLQKPGQSPNLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYFCSQGTHVPYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: X
Mutation: NULL
| >5MO9_X|Chain C[auth X]|BDNF/NT-3 growth factors receptor|Homo sapiens (9606) MGSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVTDKT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: VAL2 GLY26 TYR27 THR28 ASP31 TYR32 GLU33 ASP52 VAL99 THR100 THR101 TRP102 ALA104 TYR105 L: HIS31 ASN33 ASN35 TYR37 TYR54 LYS55 ASN58 ARG59 PHE60 SER61 X: GLN316 TRP317 PHE318 TYR319 ASN320 GLY321 ALA322 ILE323 LEU324 ASN325 ASP358 THR360 ILE362 LYS364 LYS369 ASP370 GLU371 LYS372 GLN373 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
