Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1579 |
| PDBID: | 5MJE |
| Chains: | B_A |
| Organism: | Vibrio cholerae, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1093/nar/gkx138 |
| Antibody | |
| Antibody: | Nb8 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Cytotoxic translational repressor of toxin-antitoxin stability system |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >5MJE_B|Chain B|Nanobody 8|Lama glama (9844) AQVQLQESGGGLVQPGGSLRLSCVVSGDRRTIYTMGWFRQAPGNQGELVATMTSSGVTTYVDSVKGRFSISRDSAEDSAKNTVSLQMNSLKPEDTAFYTCYEESRRPLGSRNTYWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >5MJE_A|Chain A|Cytotoxic translational repressor of toxin-antitoxin stability system|Vibrio cholerae (666) GSSHHHHHHSSGLVPRGSHMKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLTIYGKNEMSDLNANQRKQLMAFMEAWRNEQS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN3 LEU6 GLN7 LEU13 THR35 PHE39 GLN41 PRO43 GLN46 GLY47 LEU49 THR52 THR96 PHE98 THR100 TYR102 GLU104 ARG106 SER111 ARG112 ASN113 THR114 TYR115 TRP116 GLY117 GLN118 GLN121 THR123 A: GLY-7 LEU-6 VAL-5 PRO-4 HIS0 MET1 LYS2 SER3 VAL4 PHE5 VAL6 GLU7 GLU12 ARG15 ASP16 ASP21 GLU22 TYR24 ARG25 LEU26 GLN28 ALA29 GLU30 MET32 LEU33 ARG75 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
