Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1577 |
| PDBID: | 5MHS |
| Chains: | ED_B |
| Organism: | Mammalian orthoreovirus 1 Lang, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.70 |
| Reference: | 10.1128/JVI.01621-16 |
| Antibody | |
| Antibody: | 5C6 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | reovirus serotype 1 outer capsid protein sigma-1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >5MHS_E|Chain E, H, I[auth N]|5C6 Fab heavy chain|Mus musculus (10090) QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYAIHWVRQSPGKGLEWLGVIWSGGSTDYNAAFISRLSISKDNFKSQVFFKMNSLQSNDTAIYYCARKEELYGYDGYLFFDVWGAGTTVTVSSAKTTAPSVYPLVPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIE |
Light Chain: D
Mutation: NULL
| >5MHS_D|Chain D, F, G|5C6 Fab light chain|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCRASKSVSTSGYSYMQWYQQKPGQPPKLLIYLASNLESGVPVRFSGSGSGTDFTLNIHPVEEEDAAIYYCQHSRELPPTFGAGTKLELKADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: NULL
| >5MHS_B|Chain A, B, C|Outer capsid protein sigma-1|Reovirus type 1 (strain Lang) (10884) GVLNQGVTSLPTYRYPLELDTANNRVQVADRFGMRTGTWTGQLQYQHPQLSWRANVTLNLMKVDDWLVLSFSQMTTNSIMADGKFVINFVSGLSSGWQTGDTEPSSTIDPLSTTFAAVQFLNNGQRIDAFRIMGVSEWTDGELEIKNYGGTYTGHTQVYWAPWTIMYPCNVR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: ARG96 LEU98 E: THR30 ASN31 TRP52 SER53 GLY54 SER56 ASP58 GLU99 GLU100 LEU101 TYR102 GLY103 TYR104 TYR107 LEU108 B: GLN371 THR373 ASN375 ALA415 VAL416 GLN417 LEU419 ASN421 GLY422 GLN423 ARG424 ASP426 THR454 GLN455 TYR457 TRP458 ALA459 PRO460 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
