Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1572 |
| PDBID: | 5MHR |
| Chains: | PO_E |
| Organism: | Reovirus sp., Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1128/JVI.01621-16 |
| Antibody | |
| Antibody: | 9BG5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Viral attachment protein sigma 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: P
Mutation: NULL
| >5MHR_P|Chain I, K, M, N, P, R|9BG5 Fab heavy chain|Mus musculus (10090) EVQLQQSGPELVKPGASMKISCKASGYSFTGYTMNWVKQSHGKNLEWIGLINPYNDGPSYNQNFKGKATLTVDKSSSTAYMELLSLTSEDSAVYYCAIHYYGISYWYFDVWGAGTTVTVSSAKTTAPSVYPLVPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIE |
Light Chain: O
Mutation: NULL
| >5MHR_O|Chain G, H, J, L, O, Q|9BG5 Fab light chain,LOC100046793 protein,MAb 110 light chain|Mus musculus (10090) EIVLTQSPTTMAASPGEKIIITCSASSSISSNYLHWYQQKPGCSPKLLIYRTSKLASGVPARFSGSGSGTSYSLTIGTMEAEDVATYYCQQGSSIYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: E
Mutation: NULL
| >5MHR_E|Chain A, B, C, D, E, F|Viral attachment protein sigma 1|Reovirus sp. (10891) GSSPNLRYPIADVSGGIGMSPNYRFRQSMWIGIVSYSGSGLNWRVQVNSDIFIVDDYIHICLPAFDGFSIADGGDLSLNFVTGLLPPLLTGDTEPAFHNDVVTYGAQTVAIGLSSGGTPQYMSKNLWVEQWQDGVLRLRVEGGGSITHSNSKWPAMTVSYPRSFT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| O: SER26 SER27 SER28 ILE29 SER30 SER31 ASN32 TYR33 ARG51 THR52 SER53 LYS54 SER66 GLY67 SER68 GLY69 THR70 SER93 P: TYR105 E: SER327 GLY328 SER329 GLY363 GLY364 ASP365 ASN389 ASP390 VAL391 VAL392 THR393 TRP417 GLU419 GLN420 GLN422 ARG427 ARG429 GLU431 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
